Labelling Review row-Online

Primary Antibodies
CD166 (ALCAM)
Clone MOG/07
1 mL, 0.1 mL lyophilized NCL-CD166 P (HIER)
The human CD166 molecule, also known as activated leukocyte cell
adhesion molecule (ALCAM), is a glycoprotein of 100 kD that functions as a
ligand for the CD6 molecule. It is the human homolog of the chicken neural
adhesion molecule, BEN/SC-1/DM-GRASP, the rat molecule, KG-CAM, and
the fish protein, neurolin. The CD166 molecule is reported to be expressed by
a subset of activated leukocytes. CD166/CD6 interactions may play a role in
the binding of T and B cells to activated leukocytes as well as in interactions
between cells of the nervous system involving neurite extension of the
neurons. The CD166 molecule is also expressed in a number of other cell
types including activated monocytes, epithelial cells, fibroblasts, neurons,
melanoma cells and also in sweat and sebaceous glands. CD166 protein
expression is reported to be upregulated in a cell line deriving from a
metastasizing melanoma. It is also reported that CD166 protein may play a
role in T cell development in the thymus.
CD168 (RHAMM)
Clone 2D6
1 mL lyophilized NCL-CD168 F P (HIER)
The CD168 molecule, also known as RHAMM/IHABP (receptor for
hyaluronic acid mediated motility/intracellular hyaluronic acid binding
protein), is a ubiquitously expressed filamentous, cytoskeletal accessory
protein. It is not, as originally reported, a cell surface receptor. However, in
some cancers, it is reported that the expression of cell surface variants of
CD168 is closely correlated with tumor progression. The CD168 molecule
plays a role in cell signalling, migration and adhesion via interactions with
hyaluronan, microtubules, actin, calmodulin and components of the
extracellular regulated kinase (erk) signalling pathway. CD168 appears to
have an important role in human sperm motility. In the brain, the CD168
molecule is reported to be expressed in the majority of neurons and in many
oligodendrocytes where it has an effect on astrocyte motility, neurite
migration and axonal growth. CD168 antigen is necessary for migration of
smooth muscle cells after wound injury and it has been associated with
adult wound fibroplasias. Reports indicate that CD168 antigen is detected at
varying levels in normal breast epithelium but in breast cancers, strong
expression has been observed particularly in well-differentiated grade 1
ductal carcinomas, whereas high grade cancers displayed significantly
lower expression. CD168 is also reported to be expressed at low frequency
in non-cancerous gastric mucosa and in 74 percent of gastric cancers
where it is associated with malignant progression.
Normal human testis: immunohistochemical staining for CD168 antigen using NCL-CD168.
Note membrane staining of spermatocytes in the seminiferous cells. Paraffin section.
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CD205 (DEC-205)
Clone 11A10
1 mL liquid NCL-L-DEC205 P (HIER)
CD205 is a 205 kD integral membrane glycoprotein homologous to the
macrophage mannose receptor and related receptors. It is a novel
multilectin, endocytic receptor that can be used by dendritic cells and
thymic epithelial cells to direct captured antigens from extracellular spaces
to a specialized antigen processing compartment.
Human thymus: immunohistochemical staining for DEC-205 using NCL-L-DEC205.
Note cytoplasmic and membrane staining of epithelial cells. Paraffin section.
CD243 (P-glycoprotein)
Clone 5B12
1 mL lyophilized NCL-PGLYm F P (HIER)
See also P-glycoprotein (CD243) on page 150.
CD246 (Anaplastic Lymphoma Kinase)
(ALK) (p80)
Clone 5A4
1 mL, 0.1 mL lyophilized NCL-ALK P (HIER)
7 mL Bond ready-to-use PA0306 P (HIER)
See also ALK (Anaplastic Lymphoma Kinase) (CD246) (p80) on page 54.
cdk-activating kinase (CAK)
Clone MO-1.1
1 mL lyophilized NCL-CAK FWO
Cyclin dependent kinase (cdk) activation is dependent on cyclin binding and
phosphorylation of a conserved threonine residue mediated by cdkactivating
kinase (CAK). Human CAK has been identified as the p40 MO15
(cdk7)/cyclin H/MAT1 complex which is localized to the nucleus in
vertebrates. DNA repair mechanisms and regulation of gene activity both
involve CAK. NCL-CAK detects the catalytic subunit p40 MO15 (cdk7).
Product Specific Information
NCL-CAK may also be used in immunoprecipitation techniques.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.