Labelling Review row-Online

Human prostatic carcinoma: immunohistochemical staining for TNF-related apoptosisinducing
ligand using NCL-TRAIL. Note cytoplasmic staining of a proportion of malignant
cells. Paraffin section.
Topoisomerase I
Clone 1D6
1 mL lyophilized NCL-TOPO I F P (HIER)
Topoisomerases are nuclear enzymes involved in a variety of cellular
activities such as chromosomal condensation, DNA replication, transcription,
recombination and segregation at mitosis. Human topoisomerase I is a
100 kD protein capable of relaxing positively and negatively supercoiled
DNA by performing a transient single-stranded nick which is then re-ligated
at the end of the reaction. It has been shown that the enzyme is located in
regions of the genome that are undergoing active RNA synthesis where it
probably reduces superhelical stresses in the DNA enabling RNA
polymerase to function properly. In normal eukaryotic cells, DNA
topoisomerase I does not show relevant fluctuations across the cell cycle,
unlike DNA topoisomerase II alpha. Both DNA topoisomerases I and II have
been found to be targets of autoantibodies in the sera of individuals with
certain autoimmune diseases eg systemic lupus erythematosus and also of
some anti-tumor drugs and antibiotics. Elevated levels of DNA
topoisomerase I, detected by 32 P transfer assays, have been reported in
colorectal tumors compared with normal colon mucosa as a result of
increased transcription or mRNA stability.
Product Specific Information
The use of phosphate-containing wash buffers or diluents with NCL-TOPO I
has an adverse effect on staining. Only Tris-containing wash buffers or
diluents should be used.
Topoisomerase II Alpha
Clone 3F6
1 mL, 0.1 mL lyophilized NCL-TOPOIIA F P (HIER) W
Topoisomerase II alpha is an essential nuclear enzyme involved in DNA
replication and is a target for many anti-cancer drugs used for cancer
therapy. Decreased expression of topoisomerase II alpha is the predominant
mechanism of resistance to several chemotherapeutic agents. A significant
variation in the range of expression of this protein has been reported in
many different tumors. Reports of the analysis of primary breast tumors have
indicated that topoisomerase II beta is more widely expressed than
topoisomerase II alpha. Topoisomerase II alpha expression and activity is
linked to the cell cycle and is associated with the proliferation status of
cells.
Human bladder tumor: immunohistochemical staining for topoisomerase II alpha using
NCL-TOPOIIA. Note intense nuclear staining of malignant cells and occasional mitotic
figures. Paraffin section.
Toxoplasma gondii P30 Antigen
Clone TP3
1 mL lyophilized NCL-TG P (HIER)
Toxoplasma gondii is a ubiquitous protozoan parasite which can infect
healthy humans, often asymptomatically, but may also cause severe
congenital defects in the fetus and life-threatening infection in
immunocompromised hosts. It has been shown that P30, also referred to as
SAG-1, the major surface antigen of Toxoplasma gondii tachyzoites is
involved in the first steps of invasion. This antigen has been reported to have
generated interest as a potential subunit for vaccine production. P30 is a
highly conserved antigen between most strains of Toxoplasma gondii.
Infected human brain: immunohistochemical staining for Toxoplasma gondii P30 antigen using
NCL-TG. Note staining of the parasites in infected areas. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies