Labelling Review row-Online

Tyrosinase
Clone T311
7 mL Bond ready-to-use PA0322 P (HIER)
For In Vitro Diagnostic Use
The biosynthesis of melanin in melanocytes involves a family of enzymes, a
key member of which is tyrosinase.
Tyrosinase deficiency is associated with various forms of albinism and in
particular oculocutaneous albinism. L-tyrosinase is the initial substrate for
melanin biosynthesis and its conversion to dopaquinone is catalyzed by
tyrosinase, whose expression is reported in melanocytes and melanomas.
Tyrosinase expression in melanocytic lesions can be assessed using
Tyrosinase (T311).
Also available as a Novocastra concentrate, refer to page 167.
Malignant melanoma: immunohistochemical staining with Bond ready-to-use Tyrosinase
(T311) using Bond Polymer Refine Detection.
Villin
Clone CWWB1
7 mL Bond ready-to-use PA0106 P (HIER)
For In Vitro Diagnostic Use
Villin and the structurally-related proteins gelsolin, fragmin and severin, all
regulate the framework and assembly of actin. Villin is mainly produced by
epithelial cells that develop a brush border. epithelial cells of the intestinal
mucosa and gall bladder, or in epithelial cells of the kidney proximal tubules
and ductuli efferentes of the testis.
Villin is also reported to be found in some epithelia which lack a brush
border but which are derived from embryonic gut such as duct cells of the
exocrine pancreas and biliary cells of the liver. In these cell types, villin is
concentrated in the apical cytoplasm. Epithelial cells of the intestinal
mucosa are continually being renewed and this involves a migration of
these cell types from the intestinal crypts to the tips of the villi, gradually
acquiring their differentiated phenotype as they do so. The maximum
production of villin occurs at the base of the villus. Villin shows tissuespecific
expression, restricted to certain epithelia and their apical domains,
thus indicating their polarity. The morphological loss of polarity of colonic
epithelial cells is reported to be one of the most significant indicators of
dysplasia or neoplasia.
Also available as a Novocastra concentrate, refer to page 170.
Colon: immunohistochemical staining with Bond ready-to-use Villin (CWWB1) using Bond
Polymer Refine Detection.
Vimentin
Clone SRL33
7 mL Bond ready-to-use PA0033 P (HIER)
For In Vitro Diagnostic Use
Eukaryotic cells contain a number of types of cytoplasmic filamentous
proteins, microtubule, microfilaments and intermediate-sized filaments (IF).
Vimentin, a 57 kD protein that is an intermediate filament is reported to be
expressed in most cells of mesenchymal origin, including fibroblasts,
endothelial cells, smooth muscle, melanocytes as well as T and B
lymphocytes.
Also available as a Novocastra concentrate, refer to page 170.
Rhabdomyosarcoma: immunohistochemical staining with Bond ready-to-use Vimentin (SRL33)
using Bond Polymer Refine Detection.
von Willebrand Factor
Clone 36B11
7 mL Bond ready-to-use PA0400 P (HIER)
For In Vitro Diagnostic Use
Human von Willebrand factor (or factor VIII-related antigen) is a 270 kD
multimeric plasma glycoprotein. It mediates platelet adhesion to injured
vessel walls and serves as a carrier and stabilizer for coagulation factor VIII.
The von Willebrand factor has functional binding domains to platelet
glycoprotein Ib, glycoprotein Ib/IIIa, collagen and heparin. Von Willebrand
factor is synthesized by endothelial cells and is reported to be expressed in
platelets, megakaryocytes and a number of tumors, including hemagiomas,
hemagiosarcomas and Kaposi's sarcomas.
Also available as a Novocastra concentrate, refer to page 123.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Bond