Labelling Review row-Online

Elastin
Clone BA-4
0.5 mL lyophilized NCL-ELASTIN P (Enzyme)
Elastin is a polymeric protein found in connective tissue which imparts the
property of elasticity to vertebrate elastic tissue. It is synthesized and
secreted as a soluble, single-chain protein (tropoelastin) which undergoes a
number of post-ribosomal modifications prior to its organization into an
elastic fiber in the extracellular space. Once secreted, tropoelastin
molecules are joined covalently via chemical modification and cross-linking
of specific lysyl residues to form the mature insoluble elastin.
Ultrastructurally, it is predominantly an amorphous material which may
change its morphology with ageing and different disease states. The
abnormal accumulation of elastic tissue in blood vessels is found in
atherosclerosis and hypertension. Genetic defects in the elastin molecule
are reported to lead to inherited diseases such as Marfan's syndrome,
pseudoxanthoma elasticum and the Bushke-Ollendorf syndrome.
Human aorta: immunohistochemical staining for elastin using NCL-ELASTIN. Note extracellular
staining within the arterial wall. Paraffin section.
Emerin
Clone 4G5
1 mL, 0.1 mL lyophilized NCL-EMERIN F P (HIER) W
Emery-Dreifuss muscular dystrophy (EDMD) is a late onset X-linked
recessive disorder characterized by slowly progressing contractures,
wasting of skeletal muscle and cardiomyopathy usually presented as heart
block. Contractures are seen in the elbows, Achilles tendons and
postcervical muscles with humero-peroneal distribution early in the course
of the disease. The STA gene, at Xq28 locus, encodes a serine-rich 34 kD
protein, emerin, which is ubiquitous in tissues and is found in highest
concentration in skeletal and cardiac muscle. Emerin is localized in the
nuclear membrane of normal muscle cells and its deficiency plays a crucial
part in the pathology of EDMD.
Product Specific Information
NCL-EMERIN is of use in the detection of the normal STA gene product.
Human skeletal muscle: immunohistochemical staining for emerin using NCL-EMERIN.
Note perinuclear staining of all cell nuclei. Paraffin section.
Endoglin (CD105)
Clone 4G11
1 mL, 0.1 mL lyophilized NCL-CD105 P (HIER)
CD105, also known as Endoglin, is an endothelial homodimeric membrane
glycoprotein containing the peptide sequence RGD which is a recognition
motif for adhesion receptors of the integrin family. It has been proposed that
endoglin is a TGF-beta receptor. CD105 antigen is reported to be expressed
on endothelial cells of capillaries, arterioles and venules in a variety of
tissues and at low levels on acute lymphoblastic and myelocytic leukemia
cells. ENDOGLIN expression may be of interest in the study of monocyte
differentiation into macrophages, studies of cellular adhesion of circulating
blood cells and in the lysis of CD105 positive cells in the presence of
complement.
Normal human ovary: immunohistochemical staining for endoglin using NCL-CD105. Note
membrane staining of cells in both the theca interna and theca externa. Paraffin section.
Endothelial Cell Marker (CD34)
Clone QBEnd/10
1 mL, 0.1 mL lyophilized NCL-END F P (Enzyme) C
1 mL liquid NCL-L-END F P (Enzyme) C
7 mL ready-to-use RTU-END F P (Enzyme)
7 mL Bond ready-to-use PA0212 P (HIER)
Refer to page 77 for further information about CD34.
Endothelin-1 Receptor (ETA)
Clone RJT24
1 mL, 0.1 mL liquid NCL-L-ETA P (HIER)
Endothelins (ET) are potent vasoconstrictive peptides originally isolated
from vascular endothelial cells. Their biological effects are mediated
through two different receptors, endothelin-1-selective endothelin receptor
(ETA) and the non-selective receptor sub-type (ETB). Analysis of mRNA by
Northern blotting reported high levels in aorta, lung, atrium, colon and
placenta with moderate levels in the cerebral cortex, cerebellum, ventricle,
kidney, adrenal glands and duodenum, whereas liver was negative. Studies
using immunohistochemistry have reported the presence of ETA in medial
smooth muscle of arteries, full-term placenta, normal and diseased gall
bladder and ovarian luteinized granulosa cells. ETA has also been reported
to have a role in gastric ulcer healing and in the development of neural-crest
derived cells.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies