Labelling Review row-Online

Primary Antibodies
CD23
Clone 1B12
1 mL, 0.1 mL lyophilized NCL-CD23-1B12 F P (HIER)
1 mL liquid NCL-L-CD23-1B12 F P (HIER) C
7 mL ready-to-use RTU-CD23-1B12 F P (HIER)
7 mL Bond ready-to-use PA0169 P (HIER)
The CD23 molecule is the low affinity IgE receptor found on B cells. It is a
membrane glycoprotein of 45 kD and is reported to be found on a subpopulation
of peripheral blood cells, B lymphocytes and on EBV-transformed
B lymphoblastoid cell lines. Expression of CD23 antigen has been reported
on monocytes and dendritic cells.
Refer to page 21 for the Bond ready-to-use format.
CD25 (Interleukin-2 Receptor)
Clone 4C9
1 mL, 0.1 mL lyophilized NCL-CD25-305 P (HIER)
7 mL Bond ready-to-use PA0305 P (HIER)
See also Interleukin-2 Receptor (CD25) on page 125.
CD27
Clone 137B4
1 mL lyophilized NCL-CD27 F P (HIER)
CD27 antigen, a member of the nerve growth factor/tumor necrosis factor
receptor superfamily, is a type I transmembrane protein consisting of a
disulphide-linked 120 kD dimer. CD27 antigen is reported to be expressed on
mature thymocytes and on the majority of human peripheral blood
T lymphocytes, on both CD4 positive and CD8 positive subsets. CD27 antigen
is also expressed on activated B lymphocytes and a proportion of resting NK
cells. Among CD4 positive cells, CD27 antigen is preferentially expressed on
unprimed CD4 positive/CD45RA positive/CD45RO negative T lymphocytes
while primed CD4 positive/CD45RA negative/CD45RO positive T lymphocytes
express low levels of CD27. During activation, the appearance of a 32 kD
soluble form results from proteolytic cleavage of the parent molecule.
Soluble CD27 antigen has also been reported in cases of B cell chronic
lymphocytic leukemia.
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Reference Range
CD29
Clone 7F10
1 mL, 0.1 mL lyophilized NCL-CD29 P (HIER)
The �1 integrins are a family of structurally-related heterodimeric molecules
and are composed of a �1 subunit (CD29 antigen) which is associated with 1
of 6 known alpha subunits. These impart the specificity to each of the
receptors and the VLA molecules which are designated according to their
alpha chain eg VLA-1 is �1/�1, VLA-2 is �2/�1. The adhesive properties of
CD29 heterodimers on T cells can be regulated by cell activation, possibly
through interactions between the cytoplasmic domain of CD29 antigen and
the cytoskeleton. CD29 antigen is reported to be expressed on most cells
including all leukocytes, although only at low levels on granulocytes. On
T cells, CD29 antigen is expressed at higher levels on memory cells than on
naive cells. The co-expression of CD4 and CD29 antigens is found in helper/
inducer subpopulation of CD4 lymphocytes. CD29 antigen is one of several
additional molecules reported to be found on the cell membrane of
hepatocytes in cases of cirrhosis, alcoholic hepatitis and hepatitis C.
Reduced expression of CD29 antigen together with the �2 integrin, CD11b,
has been reported on peripheral blood lymphocytes from Graves' disease
patients.
Human brain, cerebellum: immunohistochemical staining for CD29 antigen using NCL-CD29.
Note membrane staining of endothelial cells. Paraffin section.
CD30
Clone JCM182
1 mL, 0.1 mL liquid NCL-L-CD30-591 P (HIER) W
7 mL Bond ready-to-use PA0790 P (HIER) New!
Clone 1G12
1 mL, 0.1mL lyophilized NCL-CD30 F P (HIER)
1 mL liquid NCL-L-CD30 F P (HIER)
7 mL ready-to-use RTU-CD30 F P (HIER)
7 mL Bond ready-to-use PA0153 P (HIER)
Clone 15B3
1 mL, 0.1 mL lyophilized NCL-CD30-365 F P (HIER)
Clone JCM182 was developed to be highly effective on formalin-fixed,
paraffin-embedded tissue sections.
The CD30 antigen is a single chain glycoprotein with a molecular weight of
120 kD. CD30 antigen is known to act as a receptor for a cytokine ligand,
CD30L, and may also play a role in the regulation of cellular growth and
transformation. CD30 antigen is reported to be expressed on the surface of
multinucleated Reed Sternberg cells, mononuclear Hodgkin's cells and in
the majority of anaplastic large cell lymphomas. The CD30 antigen is
expressed in non-Hodgkin's lymphoma and virally transformed cells, eg
EBV-transformed B cells. Most T cell lymphomas are reported not to
express CD30 antigen, with the exception of some pleo-morphic T cell
lymphomas.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range