Labelling Review row-Online

Deleted in Colorectal Cancer Protein
Clone DM51
1 mL lyophilized NCL-DCC P (HIER)
The deleted in colorectal cancer (DCC) gene located on chromosome 18 is a
tumor suppressor gene that encodes a transmembrane protein structurally
similar to NCAM. The highest reported expression of this protein can be
found in axons of the central and peripheral nervous systems where it
functions as a netrin receptor required for the guidance of the developing
axons. The DCC gene is reported to be expressed in most epithelial tissues
where the protein may participate in the regulation of cell to cell or cell to
substratum interaction. In normal colon, DCC expression is restricted to the
mucosa with intense granular cytoplasmic staining in the crypts, particularly
in the goblet cells. Altered DCC expression may be the result of allelic loss
which is reported to occur in more than 70 percent of colorectal
carcinomas, localized mutations, aberrant splicing of transcripts or allelespecific
loss of transcripts. The DCC gene has also been reported to be
inactivated in pancreatic, gastric, breast, prostatic and brain cancers and
also in some leukemias. The expression of DCC protein is reduced in these
cancers by 36 to 50 percent. In astrocytic tumors and colorectal carcinomas
reduced expression of DCC protein is reported.
Human colonic adenocarcinoma: immunohistochemical staining for deleted in colorectal
cancer protein using NCL-DCC. Note granular cytoplasmic staining of malignant epithelial
cells. Paraffin section.
Deleted in Pancreatic Cancer Locus 4
Protein
Clone JM56
1 mL lyophilized NCL-DPC4 P (HIER)
Deleted in pancreatic cancer locus 4 (DPC4) is a tumor suppressor gene
reported to be frequently mutated or deleted in pancreatic and metastatic
colon cancers. DPC4, also known as Smad4, acts as a cofactor that binds
transforming growth factor-beta receptor-activated Smad2 and Smad3
generating transcriptional complexes which translocate to the nucleus to
participate in sequence-specific DNA-binding and transcriptional activation.
Mutation or deletion of the DPC4 gene is reported in 50 percent of
pancreatic ductal adenocarcinomas and a subset of acute myelogenous
leukemias, biliary tract carcinomas, ovarian, colon and breast cancers. The
expression of DPC4 protein has been reported to be a sensitive and specific
marker for DPC4 gene alterations in pancreatic carcinomas. Loss of DPC4
expression occurs late in the neoplastic progression which leads to the
development of infiltrating pancreatic cancer when it is histologically
recognizable as a carcinoma. The continued expression of DPC4 protein is
reported in pancreatic intraductal papillary mucinous neoplasms (IPMN)
and suggests genetic differences in tumorigenesis from ductal carcinomas.
Desmin
Clone DE-R-11
1 mL, 0.1 mL lyophilized NCL-DES-DERII F P (Enzyme) W
1 mL liquid NCL-L-DES-DERII F P (Enzyme) W
7 mL ready-to-use RTU-DES-DERII F P (Enzyme)
7 mL Bond ready-to-use PA0032 P (HIER)
Product Specific Information
NCL-DES-DERII reacts with an 18 kD rod piece of the intermediate filament
protein desmin (53 kD) in muscle cells. The antibody does not appear to
recognize other intermediate filament proteins. In normal tissues, Clone DE-
RII reacts with both striated (skeletal and cardiac) and smooth muscle cells.
The labeling is confined to the Z bands in skeletal and cardiac muscle giving
a characteristic striated appearance.
Refer to page 27 for the Bond ready-to-use format.
Normal human small intestine: immunohistochemical staining for desmin using
NCL-DES-DERII. Note cytoplasmic staining of muscle cells in the muscularis externa.
Paraffin section.
DOG-1
Clone K9
1 mL, 0.1 mL liquid NCL-L-DOG-1 P (HIER)
7 mL Bond ready-to-use PA0219 P (HIER)
Reference Range
DOG-1, a 986 amino acid protein of unknown function, is expressed
predominantly on the plasma membrane of gastrointestinal stromal tumors
(GISTs) and is rarely expressed in other soft tissue tumors, which, due to
appearance, can be confused with GISTs. Immunoreactivity for DOG-1 has
been reported to be found in 97.8 percent of scorable GISTs, including all KIT
negative GISTs. Reactivity for DOG-1 has been suggested to aid in the
identification of GISTs, including Platelet-Derived Growth Factor Receptor
Alpha mutants that fail to express KIT antigen.
Product Specific Information
The use of PBS-based diluents may result in increased background staining.
Refer to page 27 for the Bond ready-to-use format.
Human gastrointestinal stromal tumor: immunohistochemical staining for DOG-1 using
NCL-L-DOG-1. Note intense membrane and cytoplasmic staining of tumor cells. Paraffin
section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies