Labelling Review row-Online

N-Cadherin
Clone IAR06
1 mL, 0.1 mL liquid NCL-L-N-Cad P (HIER)
N-Cadherin is a member of the cadherin family of calcium dependent cell
adhesion molecules. The classical cadherins include the E, N, R, P and VE-
Cadherins which are believed to be expressed in a tissue specific manner.
The classical cadherins have a characteristic structure comprising an extra
cellular calcium-binding domain, consisting of five repeats, a
transmembrane domain and a highly conserved cytoplasmic domain, which
mediates interactions with cytoskeletal components of the cell via
interactions with intracellular proteins including the catenins. Cadherins
play an important role in cell-cell adhesion, and are implicated in
segregation and aggregation of tissues during development. N-Cadherin is
reported to be expressed in various cell types including neural, myocardial
and mesenchymal cells. During tumor progression increased N-Cadherin
expression concomitant with the loss of E-Cadherin expression is one of the
features of the epithelial to mesenchymal transition, which is associated
with increased tumor invasion and poor prognosis. N-Cadherin has been
proposed as a useful marker in a panel of antibodies to differentiate
mesotheliomas from adenocarcinomas.
Human testes: immunohistochemical staining for N-Cadherin using NCL-L-N-Cad. Note
cytoplasmic and membrane staining of sertoli cells. Paraffin section.
NCAM (CD56)
Clone CD564
1 mL, 0.1 mL lyophilized NCL-CD56-564 P (HIER)
Clone 1B6
1 mL, 0.1 mL lyophilized NCL-CD56-1B6 P (HIER) W
1 mL liquid NCL-L-CD56-1B6 P (HIER) W
7 mL ready-to-use RTU-CD56-1B6 P (HIER)
See also CD56 (NCAM) on page 81.
Nerve Growth Factor Receptor (gp75)
Clone 7F10
1 mL, 0.1 mL lyophilized NCL-NGFR P (HIER)
Nerve growth factor receptor (NGFR) is a member of the nerve growth factor
(NGF) tumor necrosis factor (TNF) superfamily of receptors. Nerve growth
factor is important for the development, differentiation and survival of neurons
and its action is mediated by the binding of two distinctive cell surface
receptors; the high-affinity NGFR (TrkA ) and the low-affinity NGFR (gp75). The
functional role of gp75 has not yet been fully elucidated. In vitro, unbound
gp75 has been shown to promote neural cell death, whereas, binding of gp75
by NGF ligand or antibody has been shown to inhibit gp75-induced cell death.
NGFR (gp75) is reported to be expressed in neuronal axons, Schwann cells
and perineural cells of peripheral nerves and in non-neural cells that includes
myoepithelial cells of breast, salivary and sweat glands, outer root sheath
cells of hair follicles, adventitia of mature blood vessels and a lymphocyte
subpopulation in the spleen and lymph node. NGFR has also been reported to
be expressed in a proportion of phaeochromocytomas, paragangliomas and
tumors of peripheral nerve sheath differentiation.
Product Specific Information
NCL-NGFR is raised to the gp75 low-affinity NGFR protein.
Human brain, nucleus of Meynert neurons in basal forebrain: immunohistochemical staining
for nerve growth factor receptor (gp75) using NCL-NGFR. Note cytoplasmic and membrane
staining of cholinergic neurons. Paraffin section.
Neuroblastoma Marker
Clone NB84a
1 mL lyophilized NCL-NB84 FPC
Neuroblastoma is a complex malignant disease in children. This tumor of the
sympathetic nervous system, derived from pathologically maturing neural
crest progenitor cells, is unique among pediatric cancers because of
spontaneous regressions and catecholamine excretions. In a major study,
Mietten M et al., indicated the NB84 monoclonal antibody to be a useful
reagent to separate neuroblastoma from other small round cell tumors. Most
of the undifferentiated neuroblastomas in their study (21 of 22) and all 83
differentiated neuroblastomas reacted with NB84, but none of these tumors
were CD99 positive. Compared with synaptophysin, NB84 was more sensitive,
although less specific, in the identification of neuroblastoma in
formaldehyde-fixed tissue. In addition to neuroblastoma, skeletal and
extraskeletal Ewing’s sarcoma and medulloblastoma showed NB84 reactivity
in approximately 20 percent of cases and 50 percent of desmoplastic
small round cell tumors showed positive cells, usually in smaller numbers
than the neuroblastomas. The NB84 reactivity was seen slightly more
commonly in morphologically defined (rosette-positive) cases of peripheral
primitive neuroectodermal tumors than in Ewing’s sarcoma. However, the
NB84 positivity did not correlate with the expression of other neural markers
(neurofilament proteins, CD57, and synaptophysin) in these tumors. All other
small round cell tumors including rhabdomyosarcomas, Wilms’ tumors, and
lymphomas were NB84 negative (Miettinen M et al., Am J Surg Pathol.
22(3):327-32 (1998)).
Product Specific Information
Enzyme pretreatment may enhance staining in some cases.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies