Labelling Review row-Online

Primary Antibodies
Human gastric carcinoma: immunohistochemical staining for minichromosome maintenance
protein 3 using NCL-MCM3. Note intense nuclear staining of proliferating tumor cells.
Paraffin section.
Minichromosome Maintenance Protein 6
Clone KAT82
1 mL, 0.1 mL liquid NCL-L-MCM6 P (HIER) W
Minichromosome maintenance protein 6 (MCM6) is one of the six members
of the MCM family, involved in the initiation of DNA replication. The binding
of MCM proteins appears to make chromatin competent for replication, as
MCM-free chromatin is unable to replicate. MCM6 is detectable in
nucleosols or bound to nuclear chromatin during the G1, S and G2 phases of
the cell cycle and bound to chromatin in the cytoplasm during mitosis.
MCM6 is reported to be undetectable during the G0 phase. Due to its role as
a replication licensing factor, MCM6 is thought to represent a marker of
proliferating cells, with a similar expression pattern to Ki67 during the cell
cycle (G1, S, G2 and M). However, reports suggest that MCM6 is expressed
during the early G1 phase, when Ki67 is undetectable, therefore suggesting
that a subset of proliferating cells in early G1 phase which are undetectable
with Ki67 antibodies may be detectable with an MCM6 antibody.
Human mantle cell lymphoma: immunohistochemical staining for Minichromosome
Maintenance Protein 6 (MCM6) using NCL-L-MCM6. Note staining of proliferating cells.
Paraffin section.
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Mismatch Repair Protein (MLH1)
Clone ES05
1 mL, 0.1 mL liquid NCL-L-MLH1 P (HIER)
7 mL Bond ready-to-use PA0610 P (HIER)
MLH1, a mismatch repair protein involved in maintaining the integrity of
genetic information, alongside MSH2, MSH6 and PMS2. During DNA
replication, strand misalignment can occur resulting in alterations to
microsatellite repeats, often referred to as microsatellite instability (MSI).
These defects in DNA repair pathways have been linked to human
carcinogenesis. Mutations in the MLH1 gene have been reported to be
found in tumors with MSI, such as some forms of colon cancer eg
Hereditary nonpolyposis colon cancer (HNPCC), a subset of sporadic
carcinomas and breast cancer. Loss of expression of MLH1 has also been
reported in acute lymphoblastic leukemia, endometrial carcinoma, gastric
carcinoma and ovarian carcinoma.
Refer to page 33 for the Bond ready-to-use format.
Human small intestine: immunohistochemical staining for MLH1 protein using NCL-L-MLH1.
Note gradient of staining through the maturing and differentiated epithelial cells of the villi and
also in a proportion of the stromal cells. Paraffin section.
Mismatch Repair Protein (MSH2)
Clone 25D12
1 mL lyophilized NCL-MSH2 P (HIER)
7 mL Bond ready-to-use PA0048 P (HIER)
Human mismatch repair protein 2 (MSH2) is involved in the initial recognition
of mismatched nucleotides during the post replication mismatch repair
process. Therefore, the loss of MSH2 function leads to the accumulation of
replication errors, which in turn may be responsible for the multiple
mutations required for multistage carcinogenesis. Mutations in mismatch
repair genes have been linked to hereditary nonpolyposis colon cancer and
to sporadic cancers which exhibit microsatellite instability. MSH2 is
reported to be expressed in the nuclei of cells from a variety of tissues
including thyroid, heart, smooth muscle and the germinal centers of
lymphoid follicles. In ileum and colon, MSH2 expression has been reported
in the crypts, the cells of which are undergoing rapid renewal. They are
responsible for the continuous production of differentiated cells which
migrate over 2 to 4 days before being sloughed into the lumen.
Refer to page 34 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range
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Reference Range
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