Labelling Review row-Online

Myeloid Marker (BM1)
Clone BM-1
1 mL lyophilized NCL-BM1 FP
See also BM1 (Myeloid Marker) on page 63.
Myeloid Marker (BM2)
Clone BM-2
1 mL lyophilized NCL-BM2 F P (HIER)
See also BM2 (Myeloid Marker) on page 63.
Myeloperoxidase
Clone 59A5
1 mL. 0.1 mL lyophilized NCL-MYELO P
7 mL Bond ready-to-use PA0491 P (HIER)
New!
Myeloperoxidase is a lysosomal enzyme found in cells of the myeloid series
which metabolises most of the hydrogen peroxide generated by activated
phagocytes. It is a major constituent of azurophilic cytoplasmic granules
that uses hydrogen peroxide to oxidise a variety of aromatic compounds and
chloride ions to hypochlorous acid (HOCI), a strong oxidant. HOCI is the most
bacteriocidal oxidant known to be produced by neutrophils. HOCI reacts
with proteins to form cytotoxic chloramines. Myeloperoxidase is reported to
be a major component in all myeloid cells, including mature granulocytes
and is a superior marker to myeloperoxidase mRNA, whose level decreases
with the maturation of the cell and is not detectable from the myelocyte
stage onwards. Myeloperoxidase is reported to be expressed in neutrophil
granulocytes and monocytes in blood, in precursors of granulocytes in the
bone marrow and in Kupffer cells of the liver.
Refer to page 36 for the Bond ready-to-use format.
Human bone marrow, granulocytic sarcoma: immunohistochemical staining for
myeloperoxidase using NCL-MYELO. Note intense cytoplasmic staining of malignant myeloid
cells. Paraffin section.
Myogenin (Myf-4)
Clone LO26
1 mL, 0.1 mL lyophilized NCL-Myf-4 P (HIER) W
1 mL liquid NCL-L-Myf-4 P (HIER) W
7 mL Bond ready-to-use PA0226 P (HIER)
Rhabdomyosarcomas are a class of myoblast-derived soft tissue sarcomas
that usually express a number of muscle-specific genes and primarily affect
children and young adults. Differentiation of myogenic cells is controlled by
a set of regulatory genes including MyoD1, myogenin, Myf-5 and Myf-6.
Myf-4 is the human homolog of myogenin. Its gene product, together with
that of Myf-3, accumulates in the nucleus of differentiated cells.
Refer to page 36 for the Bond ready-to-use format.
Human rhabdomyosarcoma: immunohistochemical staining for Myf-4 protein using
NCL-L-Myf-4. Note staining of a proportion of tumor cell nuclei. Paraffin section.
Myoglobin
Clone MYO18
1 mL, 0.1 mL lyophilized NCL-MYOGLOBIN PW
7 mL Bond ready-to-use PA0727 P (HIER)
Myoglobin is a cytoplasmic, single chain polypeptide of 153 amino acids that
contains a single heme group. Myoglobin is reported to be expressed in
skeletal and cardiac muscle but not in smooth muscle and functions as an
oxygen transporting pigment.
Refer to page 36 for the Bond ready-to-use format.
Human adult skeletal muscle: immunohistochemical staining for myoglobin using
NCL-MYOGLOBIN. Note cytoplasmic staining of muscle fibers. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies