Labelling Review row-Online

Kappa Probe
5.5 mL PB0645 P
For In Vitro Diagnostic Use
Application
Kappa Probe is used in conjunction with Lambda Probe for the detection of
antibody producing B cells in formalin-fixed, paraffin-embedded tissue.
B cell neoplasms are thought to arise from a single transformed cell
(monoclonal), whereas reactive states result in proliferation of a number of
B cells (polyclonal). Since immunoglobulins from the same B cell contain
either Kappa or Lambda light chains, light chain restriction or monoclonality
can be used to make the distinction between reactive and neoplastic B cell
proliferations.
Components
Ready-to-use fluorescein-conjugated oligonucleotide probe directed to
Kappa light chain messenger RNA in formalin-fixed, paraffin-embedded
tissue. Optimized for use with Bond Polymer Refine Detection (DS9800) and
Anti-Fluorescein Antibody (AR0833/AR0222).
Human tonsil: in situ hybridization for kappa mRNA using Kappa Probe, Anti-Flourescein
Antibody and Bond Polymer Refine Detection.
Lambda Probe
5.5 mL PB0669 P
For In Vitro Diagnostic Use
Components
Lambda Probe is used in conjunction with Kappa Probe for the detection of
antibody producing B cells in formalin-fixed, paraffin-embedded tissue. B cell
neoplasms are thought to arise from a single transformed cell (monoclonal),
whereas reactive states result in proliferation of a number of B cells
(polyclonal). Since immunoglobulins from the same B cell contain either Kappa
or Lambda light chains, light chain restriction or monoclonality can be used to
make the distinction between reactive and neoplastic B cell proliferations.
Application
Ready-to-use fluorescein-conjugated oligonucleotide probe directed to
Lambda light chain messenger RNA in formalin-fixed, paraffin-embedded
tissue. Optimized for use with Bond Polymer Refine Detection (DS9800) and
Anti-Fluorescein Antibody (AR0833/AR0222) on the Bond system.
Human Tonsil: in situ hybridization for lambda mRNA using Lambda Probe, Anti-Flourescein
Antibody and Bond Polymer Refine Detection.
RNA Negative Control Probe
5.5 mL PB0809 P
For In Vitro Diagnostic Use
Components
RNA Negative Control Probe is a single oligonucleotide, designed from
zebra fish DNA and analyses using Basic Local Alignment Search Tool
(BLAST) analysis to confirm that the sequence bears no homology with any
human sequences. Optimized for use with Bond Polymer Refine Detection
(DS9800) and Anti-Fluorescein Antibody (AR0833/AR0222).
Application
The RNA Negative Control Probe is generated with a fluorescein label using
the same procedures as applied to the other oligonucleotide probes that are
used in the detection of RNA on Bond. Therefore, RNA Negative Control
Probe is ideal as a negative control probe for RNA ISH on Bond.
RNA Positive Control Probe
5.5 mL PB0785 P
For In Vitro Diagnostic Use
Components
Ready-to-use fluorescein-conjugated oligonucleotide probe directed to the
Poly(A) tail of messenger RNA in formalin-fixed, paraffin-embedded tissue.
Optimized for use with Bond Polymer Refine Detection (DS9800) and Anti-
Fluorescein Antibody (AR0833/AR0222).
Application
RNA is very susceptible to degradation by RNases, therefore, the RNA
Positive Control Probe is ideally used as a screening tool to detect the
preservation of mRNA in cells.
Staining with the RNA Positive Control Probe should result in dark brown
nuclear staining with some cytoplasmic staining, depending on the
translational activity of the cell.
Stringency Wash
6.25 mL AR0633 P
For In Vitro Diagnostic Use
Components
The Stringency Wash Solution is a formamide mixture used with the Bond
DNA Probes. This solution reduces non-specific hybridization of DNA
probes.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissue sections. The Stringency Wash Solution is intended
for use with biotin conjugated DNA probes to reduce non-specific DNA
hybridization in formalin-fixed, paraffin-embedded tissue using the automated
Bond system.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
/47
Bond