Labelling Review row-Online

Primary Antibodies
Transforming Growth Factor Beta
Clone TGFB17
1 mL, 0.1 mL lyophilized NCL-TGFB P (HIER)
Transforming growth factor beta (TGFB) is a potent cell regulatory
polypeptide homodimer of 25 kD. It variably affects cell growth, differentiation
and other aspects of cellular metabolism such as extracellular matrix
production. Its effect depends upon the cell type and other growth factors
present but in general, TGFB inhibits the growth of epithelial cells and
stimulates the growth of mesenchymal cells. Most breast lesions, benign
and malignant, involve abnormal proliferation and altered architecture of
stromal and/or epithelial elements. Inflammatory cells present in the earliest
lesions of progressive systemic sclerosis (PSS) are reported to release
TGFB possibly resulting in chemotactic recruitment of additional chronic
inflammatory cells. Platelets, a rich source of TGFB, are known to exhibit
aggregability and may contribute to the etiology of PSS.
Human breast carcinoma: immunohistochemical staining for transforming growth factor beta
using NCL-TGFB. Note cytoplasmic staining of tumor cells. Paraffin section.
Transforming Growth Factor Beta
Receptor (Type 1)
Clone 8A11
1 mL, 0.1 mL lyophilized NCL-TGFBR1 P (HIER)
Transforming growth factor beta (TGFB) is a potent cell regulatory
polypeptide homodimer of 25 kD. It variably affects cell growth, differentiation
and other aspects of cellular metabolism such as extracellular matrix
production. Its effect depends upon the cell type and other growth factors
present but in general, TGFB inhibits the growth of epithelial cells and
stimulates the growth of mesenchymal cells. Most breast lesions, benign
and malignant, involve abnormal proliferation and altered architecture of
stromal and/or epithelial elements. Platelets, a rich source of TGFB, are
known to exhibit aggregability and may contribute to the etiology of PSS.
Human colon, ulcerative colitis: immunohistochemical staining for transforming growth factor
beta receptor (type 1) using NCL-TGFBR1. Note membrane staining of a proportion of epithelial
cells and lymphocytes. Paraffin section.
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Troponin Antibodies
Clone 1A2
1 mL lyophilized troponin C NCL-TROPC P
Clone T1/61
1 mL lyophilized troponin C NCL-TROPT F P (Enzyme) W
Troponin comprises three protein subunits, troponin C, troponin I and
troponin T. It is a contractile protein which comprises 5 percent of muscle
proteins. Troponin C, an 18 kD protein, binds calcium and is responsible for
regulating the process of thin filament activation during skeletal muscle
contraction. Troponin I, a 21 kD protein, is the inhibitory subunit of the
complex and troponin T is responsible for binding the troponin subunits to
tropomyosin, a 66 kD protein that links the troponin complex to the actin
helix. The troponin C gene is reported to be expressed in three distinct
striated muscle lineages; cardiac myocytes, embryonic fast skeletal
myotubules and adult slow skeletal myocytes. Reports have indicated that
cardiac myofibers from cardiomyopathic rodent models display decreased
Ca2+ sensitivity and that this property is a result of an alteration in the
troponin/tropomyosin regulatory complex in the fibers.
Product Specific Information
NCL-TROPT reacts with human and chicken fast muscle troponin, but not
slow muscle troponin T.
Human skeletal muscle: immunohistochemical staining for troponin T using NCL-TROPT.
Note intense staining of fast muscle fibers. Paraffin section.
Tuberin
Clone 3B4
1 mL lyophilized NCL-TUBERIN P (HIER)
Tuberin is the 180 kD protein product of the tuberous sclerosis (TSC-2) gene
located on chromosome 16. Tuberous sclerosis is an autosomal disorder
known as phakomatosis which is characterized by the widespread development
of benign growths, usually described as hamartomata, in many tissues
and organs. Tuberin is widely expressed at low levels in most human
tissues, with increased expression in cortical neurons, cerebellar Purkinje
cells, motor neurons of the spinal cord, pancreatic islet B cells, cardiac
muscle and small blood vessels of the kidney and skin.
Human cerebellum: immunohistochemical staining for tuberin using NCL-TUBERIN.
Note cytoplasmic staining of Purkinje cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.