Labelling Review row-Online

Primary Antibodies
MAGE-1
Clone 6C1
1 mL lyophilized NCL-MAGE-1 P (HIER)
The human MAGE gene products are recognised by major
histocompatability complex-restricted cytotoxic T lymphocytes. MAGE-1,
also known as tumor rejection antigen, is a target for immunotherapy in
patients with hepatocellular carcinoma (HCC). MAGE-1 is reported to be
expressed in about 60 per cent of HCC cases. Other studies utilising reverse
transcriptase-PCR and southern blot hybridisation techniques have reported
MAGE genes to be expressed in malignant tumors and pre-cancerous
lesions but not in benign tumors.
Maspin
Clone EAW24
1 mL, 0.1 mL lyophilized NCL-MASPIN P (HIER)
Maspin, or mammary-specific serpin, is a tumor suppressor protein of 42 kD
that belongs to the serine proteinase inhibitor (serpin) family. It is reported to be
expressed in normal breast and prostatic epithelial cells but is downregulated
in carcinomas derived from these cell types. The expression of maspin is
controlled at the transcriptional level by a combination of elements including
Ets, AP-1 and p53. The tumor suppressor activity of maspin may depend on its
ability to inhibit angiogenesis. In breast myoepithelial cells, maspin is
predominantly a soluble cytoplasmic protein which associates with secretory
vesicles and is present at the cell surface. The loss of maspin in breast tumors
is reported to be a progressive process and expression decreases with
increasing malignancy of primary tumors and is absent from lymph node and
distant metastases. In rats, maspin mRNA has been detected in mammary
gland, vagina, bladder, thymus, small intestine, ventral prostate, seminal
vesicles and thyroid, but is absent from heart, lung, liver, brain and kidney.
Human prostate: immunohistochemical staining for maspin using NCL-MASPIN. Note
cytoplasmic and nuclear staining of normal glandular basal cells. Paraffin section.
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Mast Cell Chymase
Clone CC1
1 mL lyophilized NCL-MCC P (HIER)
Chymase is an enzyme found in human mast cells and acts as a mediator of
inflammation and matrix remodelling. Mast cells are present in most human
tissues and have themselves been implicated in angiogenesis, inflammation
and fibrosis. Mast cells are not a single cell type but represent a highly
heterogenous population. Subpopulations differ in their responsiveness to
various secretagogues, their susceptibility to pharmacological control by
anti-allergic drugs and also the extent to which they may be histologically
stained using basic dyes. Mast cells may contain both chymase and
tryptase in their secretory granules (MC TC ) or tryptase only (MC T ) without
chymase. The MC TC population normally predominates at connective tissue
sites and is also most abundant in skin, heart, gastrointestinal submucosa
and respiratory submucosa tissues. The MC T cells are most numerous in
mucosal tissues. Chymase, one of the major secretory products of MC TC
cells, may alter cytokine bioavailability by activating the interleukin-1b (IL-
1b) precursor, degrading IL-4 and liberating membrane-bound stem cell
factor. It could also participate in matrix remodelling by activating
procollagenase and control blood flow by generating angiotensin II. In
animal models, chymase has also been shown to increase microvascular
permeability and promote the accumulation of inflammatory cells.
Human tonsil: immunohistochemical staining for mast cell chymase using NCL-MCC.
Note intense cytoplasmic staining of mast cells. Paraffin section.
Mast Cell Tryptase
Clone 10D11
1 mL, 0.1 mL lyophilized NCL-MCTRYP-428 P
7 mL Bond ready-to-use PA0019 P
Mast cells contain a number of preformed chemical mediators such as
histamine, chymase, carboxypeptidase and proteolytic tryptase. A
substantial quantity of tryptase is reported to be found in mast cells of skin
and lung and suggests this enzyme plays a major role in mast cell mediated
events. In vitro studies indicate tryptase can cleave C3 to form C3a
anaphylatoxin, inactivate fibrinogen as a coaguable substrate for thrombin
and activate latent collagenase. Models of allergenic disease in the skin,
nose and lung have each indicated elevated tryptase levels. Human mast
cell tryptase has been reported to be implicated as a mediator of
inflammation. Mast cell degranulation in the gut causes mucus secretion,
mucosal edema, increased gut permeability and may be responsible for
some of the symptoms and signs of inflammatory bowel disease.
Refer to page 33 for the Bond ready-to-use format.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.