Labelling Review row-Online

Primary Antibodies
CA19-9 (Sialyl Lewis a )
Clone C241:5:1:4
1 mL lyophilized NCL-CA19-9 F P (HIER)
1 mL liquid NCL-L-CA19-9 F P (HIER)
7 mL Bond ready-to-use PA0424 P (HIER)
CA19-9 is an epitope on the sialylated Lewis a carbohydrate structure.
Sialylated Lewis a plays a role in cell adhesion by acting as a functional
ligand for the inducible adhesion molecule E-selectin. CA19-9 and CA50
(carcinoma associated mucin antigen) are useful serum markers in the
diagnosis and follow up of gastrointestinal and pancreatic cancers. In
carcinoma of the pancreas, it is reported that the immunohistochemical
expression of both CA19-9 and CA50 correlates with tumor differentiation
where the strongest staining is observed in well differentiated tumors.
These two markers are also reported in a number of benign lesions such as
chronic pancreatitis.
Product Specific Information
Clone C241:5:1:4 reacts specifically with Sialyl Lewisa - containing glycolipids,
showing no crossreaction with Lewisa , Lewisb , or other structurally related
molecules. The epitope recognized by NCL-L-CA19-9 is designated CA19-9
and is similar to CA50 (carcinoma associated mucin antigen).
Refer to page 16 for the Bond ready-to-use format.
Normal human colon: immunohistochemical staining for Sialyl Lewis a antigen using
NCL-L-CA19-9. Note extracellular-associated staining of colonic epithelial cells. Paraffin
section.
CA125 (Ovarian Cancer Antigen)
Clone Ov185:1
1 mL lyophilized NCL-CA125 F P (HIER)
1 mL liquid NCL-L-CA125 F P (HIER)
7 mL ready-to-use RTU-CA125 F P (HIER)
7 mL Bond ready-to-use PA0539 P (HIER)
CA125 antigen is usually associated with ovarian epithelial malignancies.
Serum assays are widely used to detect this protein in the monitoring of
ovarian cancers. CA125 antigen may also be detected by immunohistochemistry
and expression has been found in neoplasms such as
seminal vesicle carcinoma and anaplastic lymphoma. CA125 antigen is not
found exclusively in malignant tumors. CA125 is also known as MUC16.
Refer to page 16 for the Bond ready-to-use format.
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Papillary carcinoma of endometrium: immunohistochemical staining for ovarian cancer
antigen using NCL-L-CA125. Note staining of the luminal surface of malignant endometrial
cells. Paraffin section.
Calbindin
Clone KR6
1 mL lyophilized NCL-CALBINDIN P (HIER)
Calbindin is a calcium-binding protein belonging to the troponin C
superfamily. It functions as a buffer of cytosolic calcium and is found in the
brain, kidney, gut and pancreatic islets. In normal brain, calbindin (28 kD)
has been identified in medium sized neurons of the neuropil of the matrix
compartment of the striatum, the woolly fiber arrangements of the globus
pallidus and the fiber structures of the pars reticula of the substantia nigra.
The normal expression of calbindin is modified in patients with progressive
supranuclear palsy, striatal degeneration and Huntingdon's disease (HD). In
HD, alterations to the dendritic arbors and spiny striatal neurons may be
visualized by immunohistochemistry for calbindin. In moderate grades of HD,
proliferative changes have been found in these areas and in severe grades,
degenerative changes have been noted. A proportion of dendritic cells
within the light zone of germinal centers are also noted to be positive for
calbindin.
Human brain, cerebellum: immunohistochemical staining for calbindin using NCL-CALBINDIN.
Note cytoplasmic staining of Purkinje cells and neuronal processes. Paraffin section.
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This catalog is not for use in the USA.