Labelling Review row-Online

More documents

Recommendations

Info

Buffers Epitope Retrieval Solution pH9 1 L pH9 (x10 Concentrate) RE7119 P (HIER) Novocastra Epitope Retrieval Solutions are intended for Heat Induced Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue sections as part of an immunohistochemical procedure. HIER using an appropriate pH solution improves the staining of some antibodies by exposing epitopes within tissue that has been masked during fixation. The development of Epitope Retrieval using heat was first reported in 1991 by Shi S-R et al. Journal of Histochemistry and Cytochemistry 39: 741-748 (1991). Since then numerous studies have been published looking at the effects of molarity, pH and heating methods on epitope retrieval. A universal HIER technique suitable for all epitopes does not exist. A combination of different heating methods and epitope retrieval solutions may be used to optimize unmasking of antigens where this technique is recommended. HIER is not recommended for all antibodies. Optimum conditions for epitope retrieval should be validated by the user, as these are dependant upon tissue, fixation and/or primary antibody. RE7119 and RE7224 are supplied as a 1 L volume, sufficient to prepare 10 L of working solution. Kidney pre-treated with Epitope Retrieval Solution pH9 (RE7119). Staining for Wilms' tumor protein using NCL-L-WT1-562. Paraffin section. / 182 For detailed information on all products please visit our website: www.leica-microsystems.com Enzyme Proteinase K (IHC) 100 mL kit RE7160-K P (Enzyme) IVD IVD Enzyme pretreatment of formalin-fixed, paraffin-embedded tissue sections improves the staining of some antibodies by exposing epitopes within tissue that have been masked during fixation. The first proteolytic enzyme employed for epitope retrieval was trypsin. More recently, proteinase K which is commonly used in in situ hybridization techniques has been reported to be of use. Product Specific Information Novocastra Enzyme Proteinase K (IHC), RE7160-K, is intended for the enzymatic pretreatment of formalin-fixed, paraffin-embedded tissue sections prior to incubation with a primary antibody in an immunohistochemical (IHC) procedure. This product can be used for epitope retrieval with Novocastra antibodies for which trypsin is recommended, known exceptions to this are NCL-C-JEJUNI, NCL-BrdU, NCL-CYCLIN D1, NCL-COLL-IIp, and NCL-CYCLIN D1-GM. This two part kit comprises 0.75 mL of Enzyme Proteinase K Concentrate, RE7126, and 100 mL of Enzyme Proteinase K Buffer, RE7127, sufficient to produce 100 mL of working strength enzyme solution. This product is used in an IHC procedure, which allows the qualitative identification by light microscopy. Epitope retrieval by enzymatic pretreatment is recommended for a limited number of antibodies. Optimum conditions for epitope retrieval should be validated by the user as these are dependent upon tissue, fixation and/or primary antibody. Liver pre-treated with Enzyme Proteinase K (RE7160-K). Staining for Cytokeratin 8/18 using NCL-L-5D3. Paraffin section. Products in this catalog are subject to regulatory approval. This catalog is not for use in the USA.
NOVOCASTRA TM ISH REAGENTS/PROBES The Novocastra ISH probe range includes Fluorescein-Conjugated Oligonucleotide probes for the qualitative detection of RNA transcripts. ISH Reagents/Probes F Frozen I Immunofluorescence E Electron microscopy P Paraffin C Flow cytometry O Other applications W Western blotting / 183 ISH Reagents/Probes
Page 1 and 2:
IHC and ISH 2010 Product Range Incl
Page 3 and 4:
Reagent products are designed prima
Page 5 and 6:
CONTENTS New Products . . . . . . .
Page 7 and 8:
BOND RTU ANTIBODIES PA0117 Bcl-2 On
Page 9 and 10:
Consumables Bond Fully Automated I
Page 11 and 12:
Bond BOND TM BOND TM READY-TO-USE P
Page 13 and 14:
THE WISE THERANOSTIC CHOICE A fully
Page 15 and 16:
Ancillary Reagents Bond Wash Soluti
Page 17 and 18:
Bond Ready-to-Use Primary Antibodie
Page 19 and 20:
Calretinin Clone CAL6 7 mL Bond rea
Page 21 and 22:
CD8 Clone 4B11 7 mL Bond ready-to-u
Page 23 and 24:
CD23 Clone 1B12 7 mL Bond ready-to-
Page 25 and 26:
CD45RO Clone UCHL1 7 mL Bond ready-
Page 27 and 28:
CDX2 Clone AMT28 7 mL Bond ready-to
Page 29 and 30:
Cytokeratin 8/18 Clone 5D3 7 mL Bon
Page 31 and 32:
Placenta: immunohistochemical stain
Page 33 and 34:
Human Chorionic Gonadotrophic Hormo
Page 35 and 36:
Mast Cell Tryptase Clone 10D11 7 mL
Page 37 and 38:
Squamous cell carcinoma: immunohist
Page 39 and 40:
Negative Control (Mouse) Clone MOPC
Page 41 and 42:
Pax-5 Clone 1EW 7 mL Bond ready-to-
Page 43 and 44:
Smooth Muscle Actin Clone �sm-1 7
Page 45 and 46:
Tyrosinase Clone T311 7 mL Bond rea
Page 47 and 48:
Bond Ready-to-Use ISH Probes Anti-B
Page 49 and 50:
Kappa Probe 5.5 mL PB0645 P For In
Page 51 and 52:
Bond Universal Covertile 100 Pack S
Page 53 and 54:
NOVOCASTRA TM PRIMARY ANTIBODIES Cr
Page 55 and 56:
Name Clone Product Code Anaplastic
Page 57 and 58:
Alpha-1-Antichymotrypsin Polyclonal
Page 59 and 60:
Alpha Smooth Muscle Actin (SMA) Clo
Page 61 and 62:
Human small intestine: immunohistoc
Page 63 and 64:
Human lymph node, follicular lympho
Page 65 and 66:
BL-CAM (CD22) Clone FPC1 1 mL, 0.1
Page 67 and 68:
Calcitonin Clone CL1948 New! 1 mL,
Page 69 and 70:
Human mesothelioma: immunohistochem
Page 71 and 72:
Cathepsin G Clone 19C3 1 mL lyophil
Page 73 and 74:
Normal human tonsil: immunohistoche
Page 75 and 76:
CD10 Clone 56C6 1 mL, 0.1 mL lyophi
Page 77 and 78:
Human bone marrow: immunohistochemi
Page 79 and 80:
Product Specific Information Using
Page 81 and 82:
CD40 Clone 11E9 1 mL, 0.1 mL lyophi
Page 83 and 84:
CD45RB Clone MEM55 1 mL lyophilized
Page 85 and 86:
Normal human rectum: immunohistoche
Page 87 and 88:
CD82 Clone 5B5 1 mL lyophilized NCL
Page 89 and 90:
CD146 (MCAM) Clone N1238 1 mL, 0.1
Page 91 and 92:
CDX2 Clone AMT28 1 mL, 0.1 mL lyoph
Page 93 and 94:
Chromogranin A Clone 5H7 1 mL, 0.1
Page 95 and 96:
Complement Component C9 Clone 10A6
Page 97 and 98:
Cyclooxygenase-2 Clone 4H12 1 mL, 0
Page 99 and 100:
Cytokeratin 10 Clone LHP1 1 mL lyop
Page 101 and 102:
Cytokeratin (5/18) Clone C-50 1 mL
Page 103 and 104:
Deleted in Colorectal Cancer Protei
Page 105 and 106:
Elastin Clone BA-4 0.5 mL lyophiliz
Page 107 and 108:
Human squamous cell carcinoma of br
Page 109 and 110:
THE RESULTS ARE CLEAR Leica Microsy
Page 111 and 112:
EZH2 (Enhancer of Zeste Homolog 2 (
Page 113 and 114:
Feline Calicivirus (capsid protein)
Page 115 and 116:
Gamma-Catenin Clone 11B6 1 mL lyoph
Page 117 and 118:
Glutathione S-Transferase (GST) Ant
Page 119 and 120:
Heat Shock Protein 27 Clone 2B4 1 m
Page 121 and 122:
Acutely infected human liver: immun
Page 123 and 124:
Human Chorionic Gonadotrophin (alph
Page 125 and 126:
Human von Willebrand Factor (Factor
Page 127 and 128:
InhibinA Clone AMY82 New! 1 mL, 0.1
Page 129 and 130:
Lambda Light Chain Clone SHL53 New!
Page 131 and 132:
Lymphocyte Activation Gene-3 Protei
Page 133 and 134: Human appendix: immunohistochemical
Page 135 and 136: Mesothelin Clone 5B2 1 mL lyophiliz
Page 137 and 138: Mismatch Repair Protein (MSH6) Clon
Page 139 and 140: Human benign nodular hyperplasia of
Page 141 and 142: Myeloid Marker (BM1) Clone BM-1 1 m
Page 143 and 144: N-Cadherin Clone IAR06 1 mL, 0.1 mL
Page 145 and 146: nm23 Protein Clone 37.6 1 mL lyophi
Page 147 and 148: p53 Protein Clone IMX25 1 mL lyophi
Page 149 and 150: p57 Protein (Kip2) Clone 25B2 1 mL,
Page 151 and 152: Human acute lymphoblastic leukemia:
Page 153 and 154: Platelet-Derived Endothelial Growth
Page 155 and 156: Human seminoma: immunohistochemical
Page 157 and 158: P-selectin (CD62P) Clone C34 1 mL l
Page 159 and 160: S-100 Clone S1/61/69 1 mL, 0.1 mL l
Page 161 and 162: SNAP-25 Clone SP12 0.5 mL lyophiliz
Page 163 and 164: Breast carcinoma showing neuroendoc
Page 165 and 166: Thyroid Peroxidase Clone AC25 1 mL
Page 167 and 168: Human prostatic carcinoma: immunohi
Page 169 and 170: Tubulin Beta II Clone KNY-379 1 mL
Page 171 and 172: Vascular Endothelial Growth Factor
Page 173 and 174: Human Wilms’ tumor: immunohistoch
Page 175 and 176: Manual Detection Systems NOVOCASTRA
Page 177 and 178: DAB Enhancer 25 mL RE7125 PO Novoca
Page 179 and 180: Miscellaneous Hapten Antibodies Clo
Page 181 and 182: NOVOCASTRA TM EPITOPE RETRIEVAL REA
Page 183: Buffers Epitope Retrieval Solutions
Page 187 and 188: Human Herpesvirus (type 8) Probe (F
Page 189 and 190: Primer Sets NOVOCASTRA TM PRIMER SE
Page 191 and 192: Corynebacterium diphtheriae 100 Tes
Page 193 and 194: ORIGIN TM Familiar and trusted Novo
Page 195 and 196: CD5 Clone 4C7 50 Tests ORG-8919 IVD
Page 197 and 198: Ki67 Clone MM1 50 Tests ORG-8772 IV
Page 199 and 200: Product Name Index PRODUCT NAME IND
Page 201 and 202: Product Code Page Unit CD1a PA0235
Page 203 and 204: Product Code Page Unit Prostatic Ac
Page 205 and 206: Product Code Page Unit Bcl-6 Oncopr
Page 207 and 208: Product Code Page Unit CD14 NCL-CD1
Page 209 and 210: Product Code Page Unit CD79a NCL-CD
Page 211 and 212: Product Code Page Unit Cytokeratin
Page 213 and 214: Product Code Page Unit Galectin-1 N
Page 215 and 216: Product Code Page Unit Lamin A/C NC
Page 217 and 218: Product Code Page Unit N-Cadherin N
Page 219 and 220: Product Code Page Unit Progesterone
Page 221 and 222: Product Code Page Unit Tumor Necros
Page 223 and 224: 221 F Frozen I Immunofluorescence E
Page 226: “With the user, for the user” L
show all

Labelling Review row-Online

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?