Labelling Review row-Online

CD82
Clone 5B5
1 mL lyophilized NCL-CD82 P (HIER)
CD82 antigen, also known as KAI1 or C33 antigen, is a member of the TM4
superfamily. It is expressed in most cell types, including B and T cells,
NK cells, monocytes, granulocytes and platelets but not in erythrocytes.
Upon lymphocyte activation, CD82 antigen expression is reported to be
strongly upregulated and, in vitro, it can transduce signals in B cells, T cells
and monocytes. The expression of CD82 antigen is reported to suppress
metastasis in tumor cells. In benign prostatic hyperplasia, the expression of
CD82 antigen was found to be uniform in the cellular membrane of glandular
epithelial cells. However, tissue from untreated prostate cancer showed
more variable expression.
Human skin, squamous cell carcinoma: immunohistochemical staining for CD82 antigen using
NCL-CD82. Note intense membrane staining of tumor cells. Paraffin section.
CD83
Clone 1H4b
1 mL, 0.1 mL lyophilized NCL-CD83 P (HIER)
CD83 antigen, a member of the immunoglobulin superfamily, is reported to
be expressed on mature and activated dendritic cells. These include
Langerhans cells in the skin, peripheral blood dendritic cells and
interdigitating reticulum cells within the T cell zones of lymphoid organs. In
early human pregnancy, decidua is reported to contain immunostimulatory
CD83 antigen positive dendritic cells. CD83 antigen is reported to be
expressed in Hodgkin's disease and can be found to be expressed in most
Reed Sternberg cells. In breast carcinoma, mature CD83 positive cells may
be found in peripheral areas amongst T cells, which resembles dendritic/T
cell clusters of secondary lymphoid organs. This is a characteristic of
ongoing immune reactions where mature and activated dendritic cells are
essential for the recruitment of activated tumor specific lymphocytes during
carcinogenesis. Some germinal center B cells and activated peripheral
lymphocytes also express CD83 antigen.
Hodgkin's disease, mixed cellularity type: immunohistochemical staining for CD83 antigen
using NCL-CD83. Note membrane staining and characteristic paranuclear hofs of Reed
Sternberg cells. Paraffin section.
CD95 (Fas)
Clone GM30
1 mL lyophilized NCL-FAS-310 F P (HIER)
See also Fas (CD95) on page 110.
CD99
Clone PCB1 New!
1 mL, 0.1 mL liquid NCL-L-CD99-187 P (HIER)
Clone 12E7
7 mL Bond ready-to-use PA0509 P
CD99 is a 32 kDa transmembrane glycoprotein, encoded by the MIC2 gene,
which is located in the pseudoautosomal region of the human X and Y
chromosomes. Recently, the MIC2 gene has been shown to encode two
distinct proteins which are produced by alternative splicing of the CD99
gene transcript and are identified as bands of 30 and 32 kDa (p30/32).
Although its function is not fully understood, CD99 has been implicated in
various cellular processes including homotypic aggregation of T cells,
upregulation of T cell receptor and MHS molecules, apoptosis of immature
thymocytes and leukocyte diapedesis. CD99 is reported to be expressed on
most human tissues including cortical thymocytes, pancreatic islets cells,
Leydig and Sertoli cells, virtually all hematopoietic cell types (except
granulocytes), peripheral blood lymphocytes, granulose cells of the ovary,
endothelial cells and basal/parabasal squamous epithelial cells. CD99
expression has been reported in a wide range of tumors, including Ewing’s
sarcoma and T cell lymphoma.
Refer to page 24 for the Bond ready-to-use format.
Human primitive neuroectodermal tumors: immunohistochemical staining for CD99 using
NCL-L-CD99-187. Paraffin section.
CD105 (Endoglin)
Clone 4G11
1 mL, 0.1 mL lyophilized NCL-CD105 P (HIER)
See also Endoglin (CD105) on page 103.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies