Labelling Review row-Online

Vascular Endothelial Growth Factor
Receptor-3
Clone KLT9
1 mL liquid NCL-L-VEGFR-3 P
VEGFR-3 (FLT4) is a receptor tyrosine kinase similar in structure to VEGFR-1
and VEGFR-2 but does not bind VEGF. However, the two known ligands have
a high degree of homology to VEGF and are known as VEGF-C and VEGF-D.
VEGFR-3 is reported to be found in many tissues including lung, intestine,
brain and placenta (syncytiotrophoblasts). Throughout embryogenesis,
VEGFR-3 mRNA is expressed in most endothelial cells, whilst being
restricted to lymphatic vessels later in development. It appears to play an
important role in the normal development of blood and lymphatic vessels. In
tumors, expression has been reported in blood capillary endothelium and
VEGFR-3 is thought to be involved in angiogenesis during tumor growth.
Human placenta: immunohistochemical staining for VEGFR-3 using NCL-L-VEGFR-3.
Note cytoplasmic staining of syncytiotrophoblasts. Paraffin section.
VE-Cadherin (CD144)
Clone 33E1
1 mL liquid NCL-L-VE-Cad P (HIER)
Vascular endothelial cadherin (VE-Cadherin) is a calcium dependant
molecule involved in the adhesion cells to the extracellular matrix. VE-
Cadherin is localized to the intracellular junctions of endothelial layers, such
as those of blood and lymphatic vessels and placenta. VE-Cadherin is
unique among the adherin proteins as it is expressed only in the endothelial
layers. VE-Cadherin has been reported to be used to identify tumors derived
from endothelial tissue.
Human angiosarcoma: immunohistochemical staining for VE-Cadherin using NCL-L-VE-Cad.
Note staining of malignant endothelial cells. Paraffin section.
Vesicular Monoamine Transporter
(VMAT) Antibodies
Clone RMT77
1 mL, 0.1 mL liquid Vesicular Monoamine Transporter 1
(VMAT1) NCL-L-VMAT1 P (HIER)
Clone NN 136
1 mL, 0.1 mL liquid Vesicular Monoamine Transporter 2
(VMAT2) NCL-L-VMAT2 P (HIER)
Vesicular monoamine transporters (VMAT1 and VMAT2) mediate
monoamine accumulation from the cytoplasm into storage organelles. They
are dependent on a vacuolar ATPase-generated proton gradient to transport
the cationic amine substrates into the storage organelle in exchange for
protons. VMAT1 is a glycoprotein, located in the membranes of secretory
granules/vesicles and is expressed in enterochromaffin (EC) cells and
adrenal chromaffin cells. The presence of secretory vesicles is reported in
tumor cells and is regarded as evidence of neuroendocrine differentiation.
Demonstration of secretory granules in tumor cells has been reported using
electron microscopy or immunohistochemistry. Specific hormone production
cannot always be demonstrated in endocrine tumors, whose origin and
biological behavior may be difficult to determine. Vesicular monoamine
transporter (VMAT2) mediates the monoamine accumulation from the
cytoplasm into storage organelles and is located in the membranes of the
secretory granules/vesicles. VMAT2 is reported to be coexpressed in all
chromaffin cells of the adrenal medulla. It is also reported to be expressed in
gastric enterochromaffin-like cells, beta cells of the pancreas, Langerhans
cells of the skin and a population of central, peripheral and enteric neurons.
Human infiltrating carcinoid of the bowel: immunohistochemical staining for vesicular
monoamine transporter 1 (VMAT1) using NCL-L-VMAT1. Note intense cytoplasmic staining
of malignant cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies