Labelling Review row-Online

Cytokeratin (5/18)
Clone C-50
1 mL lyophilized NCL-C50 F P (HIER)
Cytokeratin 18 is reported to be expressed by simple epithelial cells and a
wide range of epithelial-derived tumors. Cytokeratin 5 is reported to be
expressed by basal cells and suprabasal cells of stratified epithelium.
Product Specific Information
NCL-C50 reacts with human cytokeratin intermediate filament proteins of 58
kD and 45 kD, identified as cytokeratins 5 and 18 respectively. However, the
recognition of cytokeratin 5 on paraffin sections using NCL-C50 may be
variable.
Human fetal small bowel: immunohistochemical staining for cytokeratins 5 and 18 using
NCL-C50. Note intense cytoplasmic staining of epithelial cells. Paraffin section.
Cytokeratin (8/18)
Clone 5D3
1 mL, 0.1 mL lyophilized NCL-5D3 F P (Enzyme) C
1 mL liquid NCL-L-5D3 F P (Enzyme) C
7 mL ready-to-use RTU-5D3 F P (Enzyme)
7 mL Bond ready-to-use PA0067 P (HIER)
In normal tissues, cytokeratins 8 and 18 are reported to be expressed in all
simple and glandular epithelium and in neoplastic tissues, they have been
reported to be expressed in adenocarcinomas and most squamous cell
carcinomas. These cytokeratins are absent from keratinizing squamous
carcinomas. Clone 5D3 reacts with human cytokeratin intermediate filament
proteins of 52.5 kD and 45 kD, identified as cytokeratins 8 and 18,
respectively.
Product Specific Information
Clone 5D3 shares similar specificities to clone CAM5.2 (Angus B et al. Journal
of Pathology. 153: 377-384 (1987)).
Refer to page 27 for the Bond ready-to-use format.
Human breast: immunohistochemical staining for cytokeratins 8 and 18 using NCL-L-5D3.
Note intense cytoplasmic staining of ductal epithelial cells. Paraffin section.
Cytokeratin (5/6/18)
Clone LP34
1 mL lyophilized NCL-LP34 F P (Enzyme) C
1 mL liquid NCL-L-LP34 F P (Enzyme) C
7 mL ready-to-use RTU-LP34 F P (Enzyme)
Cytokeratins 5, 6 and 18 are reported to be expressed in a broad range of
human epithelial tissues, from simple glandular epithelia to stratified
squamous epithelia. These include epithelial cells that are ectodermal,
mesodermal, or endodermal in origin. These cytokeratins have been
reported to be expressed in tumor cells of epithelial origin and less
commonly of mesothelial origin. Non-epithelial tumors such as lymphomas
do not express these cytokeratins.
Product Specific Information
Clone LP34 reacts with human cytokeratin intermediate filament proteins 5, 6
and 18 on frozen tissue. The recognition of cytokeratin 18 on paraffin sections
using clone LP34 may be variable.
Cytokeratin, Multi
Clone AE1, Clone AE3 cocktail
1 mL, 0.1 mL lyophilized NCL-AE1/AE3 F P (HIER)
1 mL liquid NCL-L-AE1/AE3 F P (HIER)
7 mL ready-to-use RTU-AE1/AE3 F P (HIER)
7 mL Bond ready-to-use PA0909 P (Enzyme)
See also Multi-Cytokeratin on page 136.
Cytokeratin, Multi (1/5/10/14)
Clone 34�E12
1 mL, 0.1 mL lyophilized NCL-CK34BE12 F P (HIER) W
7 mL ready-to-use RTU-CK34BE12 F P (HIER)
7 mL Bond ready-to-use PA0134 P (Enzyme)
See also Multi-Cytokeratin 1/5/10/14 on page 137.
Cytokeratin, Multi (4/5/6/8/10/13/18)
Clone C-11
1 mL lyophilized NCL-C11 F P (HIER)
See also Multi-Cytokeratin (4/5/6/8/10/13/18) on page 137.
(4/5/6/8/10/13/18).
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies