Labelling Review row-Online

Human squamous cell carcinoma of breast: immunohistochemical staining for epidermal
growth factor receptor using NCL-L-EGFR-384. Note intense membrane staining of tumor
cells. Paraffin section.
Epithelial Membrane Antigen
Clone GP1.4
1 mL, 0.1 mL lyophilized NCL-EMA FP
1 mL liquid NCL-L-EMA FP
7 mL ready-to-use RTU-EMA FP
7 mL Bond ready-to-use PA0035 P (HIER)
Epithelial membrane antigen (EMA), also known as episialin, is reported to
be expressed in a variety of normal and neoplastic epithelia. It has been
reported that markers to CD45 (LCA) when used in conjunction with markers
to EMA are useful in labelling cells of lymphoid origin whereas the combination
of anti-cytokeratin antibodies together with EMA is useful to
characterize cells of epithelial origin. EMA is also notably described to be
expressed in a subset of Hodgkin’s lymphomas.
Refer to page 28 for the Bond ready-to-use format.
Epithelial-Related Antigen
Clone MOC-31
1 mL lyophilized NCL-MOC-31 F P (HIER)
NCL-MOC-31 reacts with an epithelial antigen of 40 kD present on most
normal and malignant epithelia. MOC-31 is reported to be assigned to a
group of antibodies known as SCLC-Cluster 2 which react with an epithelial
antigen determined at the Second International Workshop on Small Cell
Lung Cancer (SCLC) Antigens. A characteristic of this antibody has been
reported (Edwards C and Oates J, Journal of Clinical Pathology. 48: 626-630
(1995)). Further testing has shown that either a pressure cooker (121°C)
method or a heated water bath method (90°C for 60 minutes) utilizing 0.01M
citrate buffer (pH6.0) provides the strongest staining patterns on formalinfixed,
paraffin-embedded tissue. It is recommended that for best results
customers try both methods as described on the data sheet.
Human lung adenocarcinoma: immunohistochemical staining for epithelial-related antigen
using NCL-MOC-31. Note membrane staining of tumor cells. Paraffin sections.
Epithelial Specific Antigen
Clone VU-1D9
1 mL, 0.1 mL lyophilized NCL-ESA F P (Enzyme) W
Reference Range
7 mL ready-to-use RTU-ESA F P (Enzyme)
Epithelial specific antigen (ESA) is a 40 kD cell surface glycoprotein. It is
reported to be expressed in the majority of human epithelial cells and is
rarely expressed in mesothelial cells.
Human appendix: immunohistochemical staining for epithelial specific antigen using NCL-ESA.
Note membrane staining of epithelial cells only. Paraffin section.
Epstein-Barr virus (early antigens)
Clone G3-E31
1 mL lyophilized Epstein-Barr virus (early antigen diffuse)
NCL-EADE31 P (HIER) W O
Epstein-Barr virus (EBV) early antigens are a group of non-structural
proteins, the synthesis of which do not require viral DNA replication. At least
two forms of early antigen have been identified on the basis of their
distribution in the cell, namely diffuse and restricted. The early antigen
diffuse is expressed during the early lytic phase of virus replication, most
notably in keratinocytes of hairy cell leukoplakia.
Product Specific Information
NCL-EADE31 recognizes EBV early antigen diffuse (50 to 52 kD), encoded by
the BMRF-1 open reading frame. NCL-EARF2 recognizes EBV early antigen
restricted (85 kD). NCL-EADE31 and NCL-EARF2 react with formalin-fixed,
paraffin-embedded cells eg EBV-transformed B95-8 and RAJI cells using
heat induced epitope retrieval (HIER) with citrate buffer (pH6.0).
Epstein-Barr virus transformed B95-8 cells: immunocytochemical staining for early antigen
diffuse non-structural protein using NCL-EADE31. Note intense staining of infected cells only
where early virus replication is underway. Paraffin-embedded cells.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies