Labelling Review row-Online

Feline Calicivirus (capsid protein)
Clone 1G9
0.5 mL lyophilized NCL-1G9 W
The Caliciviridae are a family of positive-stranded RNA viruses of unique
morphology characterized by a series of cup-like depressions on the
surface of the virus. Feline Calicivirus (FCV) is a ubiquitous pathogen of cats
producing a variety of clinical symptoms, including oral ulceration, upper
respiratory tract infection and polyarthritis. FCV has a genome of 7.7kb
which encodes several proteins.
Product Specific Information
NCL-1G9 detects one of these, a capsid protein of 62 kD.
Western blot: detection of feline Calicivirus (FCV) capsid protein (62 kD) using NCL-1G9. Lane A,
molecular weight markers. Lane B, CRFK cells infected with FCV immunoblotted with NCL-1G9.
Fibronectin
Clone 568
1 mL, 0.1 mL lyophilized NCL-FIB FP
Fibronectins are glycoproteins composed of two 200 kD disulfide-linked
subunits. They are found in basement membranes and in the extracellular
connective tissue matrix. Fibronectins are bound to the surface of cells by
members of a family of cellular adhesion molecules, the integrins. A number
of fibronectin isotypes exists as a result of multiple splicing of mRNA,
producing a glycoprotein of numerous domains and repeat sequences.
These domains correlate with the binding of bacteria, cells, collagen,
heparin and a variety of other macromolecules. Cellular fibronectin has
been reported to be widely expressed in the stroma of many malignant
tumors.
Product Specific Information
NCL-FIB is specific for the cell attachment domain of human fibronectin.
Enzyme pretreatment may enhance staining in some cases.
Filaggrin
Clone 15C10
1 mL lyophilized NCL-FILAGGRIN P (HIER)
Filaggrins are an important class of the intermediate filament-associated
proteins which interact with keratin intermediate filaments (IFs) of terminally
differentiating mammalian epidermis. A precursor molecule of filaggrin,
profilaggrin, accumulates in the epidermis as keratohyalin granules which,
in mouse, is phosphorylated and incapable of interaction with IFs. At the
time of terminal differentiation, the precursor is proteolytically processed by
excision of the linker to individual filaggrin molecules which are then able to
interact with keratin IFs. Filaggrins exhibit wide species variations and their
aberrant expression has been reported in a number of human keratinizing
disorders such as parakeratosis, psoriasis and molluscum contagiosum.
Filaggrin also appears to be a target molecule for rheumatoid arthritisspecific
auto-antibodies in humans.
Normal human skin: immunohistochemical staining for filaggrin using NCL-FILAGGRIN. Note
intense cytoplasmic staining of terminally differentiated keratinocytes. Paraffin section.
Filamin
Clone PM6/317
1 mL lyophilized NCL-FIL F P (HIER) W
Filamin functions as a crosslinking protein forming a flexible link between
two actin filaments in muscle. It is composed of two identical polypeptide
chains each joined to the other at one end, with an actin binding site at the
other.
Product Specific Information
NCL-FIL cross-reacts with rabbit, chicken, guinea pig and rat filamin.
Western blot: detection of filamin protein (250 kD) using NCL-FIL. Lane A, Rainbow TM molecular
weight markers (Amersham Life Science). Lane B, MRC-5 cells immunoblotted with NCL-FIL.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies