Labelling Review row-Online

Bond Ready-to-Use ISH Probes
Anti-Biotin Antibody
7.5 mL ready-to-use AR0584 P
For In Vitro Diagnostic Use
Components
Anti-Biotin Antibody is a purified anti-biotin, IgG1 isotype. It is supplied
ready-to-use.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissue sections. ISH probes used for detection of DNA on
the Bond contain a biotin label. The Anti-Biotin Antibody allows the linking of
the probe with the detection reagents and consequently visualization of a
chromogenic product by light microscopy.
Anti-Fluorescein Antibody
3.75 mL AR0833 P
15 mL AR0222 P
For In Vitro Diagnostic Use
Components
Anti-Fluorescein Antibody is a purified IgG fraction of a mouse monoclonal
antibody. It is supplied ready-to-use.
Application
In situ hybridization (ISH) allows the detection and visualization of specific
nucleic acids in tissues sections. ISH probes used for the detection of
mRNA on Bond contain a fluorescein label. The Anti-Fluorescein Antibody
allows linking of the oligonucleotide probe with the detection reagents, and
consequently, visualization of a chromogenic product by light microscopy.
CMV Probe
5.5 mL PB0614 P
For In Vitro Diagnostic Use
Components
Ready-to-use fluorescein-conjugated oligonucleotide probe directed to human
cytomegalovirus (CMV) early gene RNA transcript in formalin-fixed, paraffinembedded
tissue. Optimized for use with Bond Polymer Refine Detection
(DS9800) and Anti-Fluorescein Antibody (AR0833/AR0222).
Application
CMV is a member of the Beta Herpes Virus family, transmitted via body fluids,
and can establish primary infection, latent infection and subsequent viral
reactivation. CMV is a common opportunistic pathogen, capable of causing
serious disease in immunocompromised individuals such as AIDS patients,
transplant patients and in neonates. Congenital CMV is a result of intrauterine
infection and although the majority of children are asymptomatic, congenital
CMV can result in sensorineural hearing loss, cognitive, motor and visual
deficits and seizures.
Human placenta: in situ hybridization for cytomegalovirus mRNA using CMV Probe,
Anti-Flourescein Antibody and Bond Polymer Refine Detection.
DNA Negative Control
6.25 mL PB0731 P
For In Vitro Diagnostic Use
Components
DNA Negative Control is a ready-to-use reagent consisting of the hybridization
solution used in the Bond DNA Probes, for use in formalin-fixed paraffin
embedded tissue. Optimized for use with Bond Polymer Refine Detection
(DS9800), Anti-Biotin Antibody (AR0584) and Stringency Wash (AR0633).
Application
Negative control probes should be run on patient tissue to confirm the
absence of background staining resulting from non-specific interactions
that would influence the test result.
DNA Positive Control
6.25 mL PB0682 P
For In Vitro Diagnostic Use
Components
The DNA Positive Control Probe is a probe designed to specifically hybridize
to the genomic ALU repeat sequences, which represent approximately 10
percent of the human genome. It is generated with a biotin label using the
same procedures as applied to the Bond DNA Probes, optimized for use with
Bond Polymer Refine Detection (DS9800), Anti-Biotin Antibody (AR0584) and
Stringency Wash (AR0633) in formalin-fixed paraffin embedded tissue.
Application
Positive control probes should be run on patient tissue to confirm that all
reagents are working correctly and to provide information on the
preservation of nucleic acids in the tissue as well as accessibility of nucleic
acids to the probe.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Bond