Labelling Review row-Online

Primary Antibodies
Human prostate: immunohistochemical staining for cytokeratin 5 using NCL-L-CK5.
Note intense cytoplasmic staining of myoepithelial cells. Paraffin section.
Cytokeratin 6
Clone LHK6B
1 mL lyophilized NCL-CK6 F
Cytokeratins are precisely regulated in tissue and little is known about the
molecular mechanisms underlying this regulation. However, the expression
pattern of cytokeratin 6 is known to be particularly complex. It is found in
hair follicles, suprabasal cells of a variety of internal stratified epithelia, in
epidermis, in both normal and hyperproliferative situations. Epidermal injury
results in activation of keratinocytes which produce and respond to growth
factors and cytokines and become migratory. Activated keratinocytes
express a specific pair of cytokeratins, 6 and 16. Furthermore, cytokeratin 6
is reported to be expressed in approximately 76 percent of head and neck
squamous cell carcinomas. The expression of cytokeratin 6 has been
particularly associated with differentiation.
Product Specific Information
NCL-CK6 reacts with the human cytokeratin intermediate filament protein
(56 kD) identified as cytokeratin 6.
Cytokeratin 7
Clone RN7
1 mL, 0.1 mL liquid NCL-L-CK7-560 P (HIER)
7 mL Bond ready-to-use PA0942 P (HIER)
Clone OV-TL 12/30
1 mL lyophilized NCL-CK7-OVTL F P (HIER/Enzyme) W
1 mL liquid NCL-L-CK7-OVTL F P (HIER/Enzyme) W
7 mL ready-to-use RTU-CK7-OVTL F P (HIER/Enzyme)
Clone RN7 was developed to produce superior staining on paraffin sections.
Cytokeratins are intermediate filament proteins present in epithelial cells.
They are expressed in a tissue-specific manner in normal organs and the
tumors that arise from them. Cytokeratin 7 belongs to the neutral basic type
B subfamily of cytokeratins. Its distribution is confined to glandular and
transitional epithelia. Cytokeratin 7 is reported to be expressed in abundance
in cultured bronchial and mesothelial cells but only at lower levels in
cultured epidermal cells. The predicted amino acid sequence of this keratin
has revealed a striking difference between this keratin and the type II
keratins expressed in epidermal cells. Cytokeratin 7 has been reported in
adenocarcinomas of the lung, breast, endometrium, ovary, thyroid as well as
in carcinomas of the bladder and chromophobe renal cell carcinoma.
Cytokeratin 7 and Cytokeratin 20 expression have been reported to show
characteristic patterns on primary and metastatic lung and colorectal
adenocarcinomas.
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Reference Range
Product Specific Information
Where clone OV-TL 12/30 can produce unwanted staining of endothelial cells,
clone RN7 does not stain these cell types. The choice of epitope retrieval,
heat or enzyme, to provide the best result with clone OV-TL 12/30 should be
determined by the user. Clones RN7, OV-TL 12/30 and LP5K react with the
human cytokeratin intermediate filament protein (54 kD) identified as
cytokeratin 7.
Refer to page 25 for the Bond ready-to-use format.
Infiltrating lobular carcinoma of breast: immunohistochemical staining for cytokeratin 7
antigen using NCL-L-CK7-560. Note intense membrane and cytoplasmic staining of malignant
cells. Paraffin section.
Cytokeratin 8
Clone TS1
1 mL, 0.1 mL lyophilized NCL-CK8-TS1 F P (HIER)
1 mL liquid NCL-L-CK8-TS1 F P (HIER)
7 mL ready-to-use RTU-CK8-TS1 F P (HIER)
7 mL Bond ready-to-use PA0567 P (HIER)
Cytokeratin 8, also known as tissue polypeptide antigen (TPA), together with
cytokeratin 18, is one of the first cytokeratins expressed in the embryo and
persists in adult tissues. Both cytokeratins, 8 and 18, are major components
of all simple epithelia but not of stratified squamous epithelia. Cytokeratin 8,
reported to be expressed in the adenocarcinomas of individuals, is also
found to be present in their sera.
Product Specific Information
Clone TS1 reacts with human cytokeratin intermediate filament protein
(52.5 kD) identified as cytokeratin 8.
Refer to page 26 for the Bond ready-to-use format.
Human pulmonary adenocarcinoma: immunohistochemical staining for cytokeratin 8 using
RTU-CK8-TS1. Note cytoplasmic staining of malignant cells. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.