Labelling Review row-Online

Primary Antibodies
Neurofilament Antibodies
Clone DA2
1 mL lyophilized Neurofilament 68 kD
NCL-NF68-DA2 F P (HIER)
Clone NR4
1 mL lyophilized Neurofilament 68 kD
NCL-NF68 F P (HIER)
Clone RT97
1 mL lyophilized Neurofilament 200 kD
NCL-NF200 FP
Clone N52.1.7
1 mL lyophilized Neurofilament 200 kD
NCL-NF200-N52 F P (HIER)
7 mL Bond ready-to-use PA0371 P (HIER)
Neurofilaments constitute the main structural elements of neuronal axons
and dendrites. Neurofilaments are composed of three major subunits
referred to as the neurofilament triplet, with molecular weights of 68 kD,
160 kD and 200 kD. Neurofilament subunits are reported to be present in
neurons, neuronal processes, peripheral nerves and sympathetic ganglion
cells. Within tumors, only neoplastic cells of neural origin or those exhibiting
neuronal differentiation, have been reported to express neurofilaments.
Refer to page 37 for the Bond ready-to-use format.
Human spinal cord, lumbar: immunohistochemical staining for the 68 kD neurofilament using
NCL-NF68-DA2. Note intense cytoplasmic staining of perikarya and neuronal axons. Paraffin
section.
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Neuron Specific Enolase
Clone 22C9
1 mL lyophilized NCL-NSE-435 PW
7 mL Bond ready-to-use PA0435 P (HIER)
Clone 5E2
1 mL lyophilized NCL-NSE2 FPW
1 mL liquid NCL-L-NSE2 FPW
7 mL ready-to-use RTU-NSE2 FP
Enolase is a glycolytic enzyme catalysing the reaction pathway between 2phosphoglycerate
and phosphoenol pyruvate. In mammals, enolase
molecules are dimers composed of three distinct subunits (�, � and �)
whereas, in rats, five forms have been found. The � subunit and � subunit
are of approximately 47 kD and 45 kD, respectively. The �� and �� enolases
are located mainly in the nervous tissue and neuroendocrine cells.
Product Specific Information
Clone 22C9 was developed to produce superior staining on paraffin sections.
Clone 22C9 reacts with the � subunit of the enolase isoenzyme. Clone 5E2
reacts with the 47 kD component of the gamma-gamma enolase isoenzyme.
Refer to page 37 for the Bond ready-to-use format.
Nitric Oxide Synthase Antibodies
Clone NOS-125
1 mL lyophilized Nitric Oxide Synthase-1
NCL-NOS-1 P (HIER)
Clone RN5
1 mL lyophilized Nitric Oxide Synthase-3
NCL-NOS-3 F P (HIER)
Human nitric oxide synthases are a family of enzymes responsible for the
synthesis of nitric oxide from L-arginine and molecular oxygen. There are at
least three nitric oxide synthases; NOS-1, also known as neuronal NOS or
nNOS, NOS-2, which is referred to as inducible NOS or iNOS and NOS-3,
also known as endothelial NOS or eNOS. As suggested by their nomenclature,
these enzymes have different cellular distribution and are subjected
to different regulatory mechanisms. NOS-3 is reported to be constitutively
expressed and produces picomolar quantities of nitric oxide (NO) which play
a role in signal transmission resulting in physiological effects. In the
gastrointestinal tract, NO is reported to play a protective role where it has
direct microbiocidal properties and acts as a first line of mucosal defence in
the stomach. The function of NO in tumor development, promotion and
progression is unclear. The effects may be both beneficial but also
detrimental to those individuals with gastric cancer where it is reported that
NO supports tumor progression through the creation of neovasculature.
Human small intestine: immunohistochemical staining for nitric oxide synthase-1 using
NCL-NOS-1. Note cytoplasmic staining of enteric ganglia. Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.