Labelling Review row-Online

Primary Antibodies
Estrogen and Progesterone Receptor
Antibodies (duo packs)
Clone 6F11 and Clone 1A6
2×1mLlyophilized NCL-ER/PGR-312d/1 F P (HIER) W
2 × 0.5 mL lyophilized NCL-ER/PGR-312d F P (HIER) W
2×1mLlyophilized NCL-ER/PGRd/1 F P (HIER) W
2 × 0.5 mL lyophilized NCL-ER/PGRd F P (HIER) W
Product Specific Information
For convenience, Leica Microsystems offer two antibodies in one pack.
NCL-ER/PGR-312d/1 and NCL-ER/PGRd/1 are 2x1mLunit alternatives to the
2 x 0.5 mL duo packs and are recommended as a more economic option for
high volume users of these antibodies.
Estrogen Receptor (beta)
Clone EMR02
1 mL lyophilized NCL-ER-beta P (HIER) W
Estrogen Receptor alpha (ER�) and beta (ER�) are the translated products
of separate genes located on different chromosomes. Although both
isoforms share a high degree of amino acid homology, the role of the
conserved domains demonstrate specific functions. The A/B region, D
domain and F domains are notably distinct in sequence. ER� is the highly
characterized estrogen receptor cloned originally from a human breast
cancer cell line with ER� more recently identified in rodents and now in
humans. ER� is reported to be expressed as multiple isoforms. ER�, unlike
ER�, is widely expressed being found in normal adult tissues of ovary,
fallopian tube, lung, kidney, brain, heart, prostate and testis.
Ets-1 Oncoprotein
Clone 1G11
1 mL, 0.1 mL lyophilized NCL-ETS-1 F P (HIER) W
The proto-oncogene c-Ets-1 is a transcription factor known to regulate
expression of a number of genes involved in extracellular matrix
remodelling. The processes of tumor invasion and metastasis are thought to
depend on the increased proteolytic activity of the invading tumor cells that
may involve matrix metalloproteinases, cathepsins B and D and plasminogen
activator in the metastatic cascade. Ets-1 interacts with the
urokinase-type plasminogen activator gene enhancer and with the
promoters of stromelysin-1 (MMP3) and collagenase-1 (MMP1) gene which
may implicate it in this process. Ets-1 is reported to be absent from normal
gastric epithelium, but is expressed in approximately 60 percent of gastric
carcinomas and oral squamous cell carcinomas. The Ets-1 proto-oncogene
is also preferentially expressed in lymphoid cells, where it is essential for
the maintenance of the normal pool of resting T and B cells. Ets-1 expression
level and distribution are differentially controlled in resting, activated and
apoptotic lymphocytes.
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Human tonsil: immunohistochemical staining for Ets-1 oncoprotein using NCL-ETS-1.
Note nuclear staining in a proportion of lymphocytes. Paraffin section.
Excitatory Amino Acid Transporter 2
Clone 1H8
1 mL lyophilized Excitatory Amino Acid Transporter 2
NCL-EAAT2 F P (HIER)
Human excitatory amino acid transporters (EAATs) are members of a family of
high affinity sodium-dependent transporter molecules that regulate
neurotransmitter concentrations at the excitatory glutamatergic synapses of
the mammalian central nervous system. It is reported that these proteins are
thought to reduce extracellular glutamate concentration, thereby modulat-ing
synaptic signalling to replenish glutamate levels and prevent glutamate
induced excitotoxicity. A decrease in glutamate transporter activity has been
associated with amyotrophic lateral sclerosis and excitotoxicity may be causal
or exacerbating in neurodegenerative diseases, including cerebral ischemia
and epilepsy. EAAT1 is reported to be prominently expressed in the cerebellum,
frontal cortex, hippocampus and basal ganglia, is a potent antagonist and also
appears to specifically block amino acid transport mediated by EAAT2.
Human brain, normal adult cerebellum: immunohistochemical staining for excitatory amino
acid transporter 2 using NCL-EAAT2. Note intense membrane staining of glial cells. Paraffin
section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.