Labelling Review row-Online
Labelling Review row-Online
Labelling Review row-Online
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Buffers Epitope Retrieval Solutions<br />
Antibody Diluent<br />
500 mL RE7133 FPO<br />
Novocastra IHC Diluent is intended for use as a diluent for Novocastra<br />
primary antibodies, Novocastra Concentrated Biotinylated Secondary<br />
Antibody, RE7108, and Novocastra Concentrated Streptavidin-HRP, RE7109,<br />
in immunohistochemical (IHC) procedures. Novocastra IHC Diluent is not<br />
intended for the reconstitution of lyophilized reagents. Volume supplied is<br />
500 mL.<br />
Normal Serum Reagents<br />
Blocking Reagent<br />
10 mL Normal Goat Serum NCL-G-SERUM FP<br />
10 mL Normal Horse Serum NCL-H-SERUM FP<br />
10 mL Normal Rabbit Serum NCL-R-SERUM FP<br />
IVD<br />
Normal serum is often used as a negative control or as a blocking reagent in<br />
immunoassays. These may be of use as ‘no primary' controls and as a<br />
diluent for primary and secondary antibody reagents. Novocastra offers<br />
these animal sera in a convenient 10 mL pack size. 200 mL of working<br />
strength diluent can be prepared, sufficient for up to 2000 slides.<br />
RUO<br />
RUO<br />
RUO<br />
Epitope Retrieval Solutions pH6<br />
1 L pH6 (x10 Concentrate) RE7113 P (HIER) IVD<br />
500 mL pH6 (x10 Concentrate) RE7114 P (HIER)<br />
Novocastra Epitope Retrieval Solutions are intended for Heat Induced<br />
Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue<br />
sections as part of an immunohistochemical procedure. HIER using an<br />
appropriate pH solution improves the staining of some antibodies by<br />
exposing epitopes within tissue that has been masked during fixation. The<br />
development of Epitope Retrieval using heat was first reported in 1991 by Shi<br />
S-R et al., Journal of Histochemistry and Cytochemistry 39: 741-748 (1991).<br />
Since then numerous studies have been published looking at the effects of<br />
molarity, pH and heating methods on epitope retrieval. A universal HIER<br />
technique suitable for all epitopes does not exist. A combination of different<br />
heating methods and epitope retrieval solutions may be used to optimize<br />
unmaskingof antigens where this technique is recommended. HIER is not<br />
recommended for all antibodies. Optimum conditions for epitope retrieval<br />
should be validated by the user, as these are dependant upon tissue, fixation<br />
and/or primary antibody. RE7113 is supplied as a 1 L volume, sufficient to<br />
prepare 10 L of working solution. RE7114 is supplied as a 500 mL volume,<br />
sufficient to prepare 5Lofworking solution.<br />
Colonic adenocarcinoma pre-treated with Epitope Retrieval Solution pH6 (RE7113). Staining for<br />
Cytokeratin 20 protein using NCL-L-CK20-561. Paraffin section.<br />
Epitope Retrieval Solutions pH8<br />
1 L pH8 (x10 Concentrate) RE7116 P (HIER)<br />
Novocastra Epitope Retrieval Solutions are intended for Heat Induced<br />
Epitope Retrieval (HIER) on formalin-fixed, paraffin-embedded tissue<br />
sections as part of an immunohistochemical procedure. HIER using an<br />
appropriate pH solution improves the staining of some antibodies by<br />
exposing epitopes within tissue that has been masked during fixation. The<br />
development of Epitope Retrieval using heat was first reported in 1991 by Shi<br />
S-R et al., Journal of Histochemistry and Cytochemistry 39: 741-748 (1991).<br />
Since then numerous studies have been published looking at the effects of<br />
molarity, pH and heating methods on epitope retrieval. A universal HIER<br />
technique suitable for all epitopes does not exist. A combination of different<br />
heating methods and epitope retrieval solutions may be used to optimize<br />
unmaskingof antigens where this technique is recommended. HIER is not<br />
recommended for all antibodies. Optimum conditions for epitope retrieval<br />
should be validated by the user, as these are dependant upon tissue, fixation<br />
and/or primary antibody. RE7116 is supplied as a 1 L volume, sufficient to<br />
prepare 10 L of working solution.<br />
F Frozen I Immunofluorescence E Electron microscopy<br />
P Paraffin C Flow cytometry O Other applications<br />
W Western blotting<br />
IVD<br />
IVD<br />
/ 181<br />
Buffers