Labelling Review row-Online

Alpha-1-Antichymotrypsin
Polyclonal
1 mL lyophilized NCL-A1ACp P
Alpha-1-Antichymotrypsin (ACT) is an early-stage acute phase protein and a
member of the serine proteinase inhibitor or serpin superfamily. The precise role
of ACT is uncertain but it is thought to act as an anti-inflammatory agent
inhibiting chymotrypsin, cathepsin G, mast cell chymase, neutrophil chemotaxis
and superoxide anion production. ACT is synthesized primarily by hepatocytes of
the liver. Lower levels of synthesis have also been discovered via
immunohistochemical analysis in mast cells, endothelial cells, breast and
intestinal epithelial cells. ACT also exists in the brain. Research has shown that it
is found in amyloid fibrils, endothelial cells and the cytoplasm of astroglial cells
in certain brain abnormalities. Further research has also shown that a major
proportion of prostate-specific antigen (PSA) in serum exists complexed to ACT.
Human malignant melanoma: immunohistochemical staining for alpha-1-antichymotrypsin
using NCL-A1ACp. Note intense cytoplasmic staining of malignant cells. Paraffin section.
Alpha-1-Antitrypsin
Polyclonal
1 mL lyophilized NCL-A1Ap F P (Enzyme) W
Alpha-1-antitrypsin is synthesized in the liver and is present in serum and
tissue fluids where it acts as an inhibitor of proteases, particularly elastase.
Its main function appears to be the neutralization of elastase released by
neutrophils during an inflammatory response. Alpha-1-antitrypsin deficiency
may result in uninhibited elastase-induced tissue destruction eg in the lung.
Alpha-1-antitrypsin deficiency is associated with panacinar emphysema and
liver disease. In the liver, alpha-1-antitrypsin deficiency may lead to
neonatal hepatitis or an individual may present in later childhood or
adulthood with cirrhosis.
Human egg yolk sac tumor: immunohistochemical staining for alpha-1-antitrypsin using
NCL-A1Ap. Note cytoplasmic staining of tumor cells. Paraffin section.
Alpha-Actinin
Clone RBC2/1B6
1 mL, 0.1 mL lyophilized NCL-alpha-ACT FW
Alpha-actinin is a rod-like cytoskeletal protein belonging to the same family
as spectrin, dystrophin and utrophin. In skeletal muscle, alpha-actinin is
located in the Z band/disc and cross-links with F-actin in this region. Muscle
tissues show the presence of abundant threadlike particles, known as
nemaline bodies, in the myofibers. Electron microscopy studies have shown
that the nemaline rods have a lattice structure similar to that of the Z discs
and the rods are thought to be lateral polymers of the Z discs.
Alpha B Crystallin
Clone G2JF
1 mL, 0.1 mL lyophilized NCL-ABCrys-512 F P (HIER) W
Alpha B crystallin is a lens protein that has some homology with the small
heat shock proteins. It is expressed in tissues such as skeletal muscle,
cardiac muscle, smooth muscle, renal tubular epithelium, Schwann cells,
glial cells, thyroid epithelium, colonic epithelium and stratified squamous
epithelium. Alpha B crystallin is reported to be found in ubiquitinated
intermediate filament inclusion bodies, such as Lewy bodies
(neurofilaments), Rosenthal fibers (glial filaments) and Mallory bodies
(cytokeratins). However, it is rarely found in neurofibrillary tangles. The role
of Alpha B crystallin in inclusion bodies is unknown, but it may function as
an accessory protein for intermediate filament aggregation. Alpha B
crystallin is reported to be expressed in various carcinomas including renal
cell carcinoma.
Human renal cell carcinoma: immunohistochemical staining for alpha B crystallin using
NCL-ABCrys-512. Note intense cytoplasmic staining of malignant cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies