Labelling Review row-Online

Complement Component C9
Clone 10A6
1 mL, 0.1 mL lyophilized NCL-CCC9 P (HIER)
Complement component C9 binds to the C5b-8 complex as the final protein
of the membrane attack complex. After binding, it undergoes a conformational
change and inserts itself into the cell membrane, forming
transmembrane channels. Complement component C9 acts in a similar way
to perforin, a pore forming protein found in cytotoxic T cells. Male and
female reproductive tissues express and synthesize complement
components, binding proteins and receptors, although the implications of
this is unclear. The detection of complement component C9 has been
reported in cases of acute myocardial damage at necropsy. Detection of
myocardial infarction or diffuse damage can be unreliable with conventional
methods of examination of the heart such as enzyme histochemistry or by
the elaborate technique of quantification of contraction band necrosis.
Human myocardium: immunohistochemical staining for complement component C9 using
NCL-CCC9. Note staining of partially necrotic myocardium and vessel walls. Paraffin section.
CPP32 (Caspase-3)
Clone JHM62
1 mL, 0.1 mL lyophilized NCL-CPP32 P (HIER) W
Cysteine protease protein (CPP)-32 is a member of the interleukin-1 betaconverting
enzyme (ICE) family of mammalian proteases which specifically
cleaves substrates at the C-terminal side of aspartic acid residues.
Members of this family have been implicated in apoptosis and CPP32
(caspase-3) is thought to act as a control mediator of programmed cell
death in mammalian cells. CPP32 is synthesized as an inactive 32 kD
proenzyme and is processed during apoptosis to its active form which is
responsible for the cleavage of poly (ADP-ribose) polymerase (PARP), actin
and sterol regulatory element binding protein (SREBP). CPP32 is reported to
be found in epithelial cells of skin, renal proximal tubules and collecting
ducts, epithelioreticular cells of the thymus and bronchial, colonic and
salivary duct epithelia. Chondrocytes, bone osteocytes, megakaryocytes,
mature neutrophils of bone marrow and plasma cells of the tonsil, lymph
node and bone marrow are also reported to express CPP32 antigen.
Cyclin A
Clone 6E6
1 mL lyophilized NCL-CYCLIN A P (HIER) W C
Cyclins are proteins that vary in abundance and are associated with and
activate cyclin dependent kinases (cdk) at different stages of the cell cycle.
Cyclin A, more commonly defined as A2, a protein of 60 kD, binds
independently to a cdc-related kinase, cdk2, in S to G2 phase and cdc2/cdk1
in G2 to M phase, leading to enzyme activation. Cyclin A is detectable in
S phase, increasing during cell cycle progression to G2 phase and may
prove useful as a marker of proliferation. Cyclin A is reported to be
expressed in normal human epidermis and various proliferative skin
diseases including psoriasis, seborrhoeic keratosis and squamous cell
carcinoma. The majority of breast tumors reported with elevated fractions of
cells expressing cyclins A, B and E have been shown to have increased
proliferative status.
Normal human colon: immunohistochemical staining for Cyclin A using NCL-CYCLIN A. Note
intense nuclear staining of a small proportion of crypt epithelial cells. Paraffin section.
Cyclin B1
Clone 7A9
1 mL, 0.1 mL lyophilized NCL-CYCLIN B1 P (HIER) W C
Cyclin B protein acts in a similar way to cyclin A, as regulatory subunits of
p34/cdc2/cdk1 affecting the G2 to M phase transition. Cyclin B expression is,
therefore, restricted to a specific short period of the cell cycle with cyclin B1
expression detected earlier and peaking in concentration before cyclin B2
expression. Cyclin B positive cells, indicated by cytoplasmic staining, in
proliferating tissue are reported to represent a subset of Ki67 positive cells.
Product Specific Information
Please note that methacarn fixation produces optimal staining.
Human breast carcinoma: immunohistochemical staining for cyclin B1 using NCL-CYCLIN B1.
Note cytoplasmic staining of tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
/93
Primary Antibodies