Labelling Review row-Online

nm23 Protein
Clone 37.6
1 mL lyophilized NCL-nm23 FPW
The nm23 gene was first identified by differential screening of mousederived
melanoma cell lines of high and low metastatic potential. Two
human homologs of the nm23 gene have been isolated and designated
nm23-H1 and nm23-H2. The products of these genes have been identified as
nucleoside diphosphate kinase A (NDPK-A) and nucleoside diphosphate
kinase B (NDPK-B), respectively. nm23-H1 and nm23-H2 are metastasissuppressor
genes implicated in the control of the metastatic process of
malignant cells.
Product Specific Information
NCL-nm23 reacts with H1 strongly and only weakly with the H2 homolog.
NS3 (Hepatitis C virus)
Clone MMM33
1 mL, 0.1 mL lyophilized NCL-HCV-NS3 F P (HIER)
See also Hepatitis C virus (NS3) on page 118.
Nucleoporin (88 kD)
Clone JRC1
1 mL lyophilized NCL-Nup88 P (HIER) W
Nuclear pore complexes are large, elaborate macromolecular structures
that mediate bidirectional nucleocytoplasmic traffic. In vertebrates, nuclear
pore complexes are made up of 50 to 100 proteins called nucleoporins (Nup).
The 88 kD nucleoporin (Nup88) is associated with a dynamic subcomplex of
oncogenic nucleoporin CAN/Nup 214. Nup88 may be regarded as a widely
distributed proliferation marker that is reported to be overexpressed in
oncogenesis and development. In cell lines, it is localized at the nuclear
membrane. In malignancies, the protein is reported to be overexpressed
with a perinuclear and cytoplasmic distribution. In hyperplasia, benign
tumors and most normal tissues, Nup88 is reported to be either weakly
expressed or undetectable.
Human breast carcinoma: immunohistochemical staining for nucleoporin (88 kD) using
NCL-Nup88. Note perinuclear and cytoplasmic staining of malignant cells. Paraffin section.
Oct-2
Clone Oct-207
1 mL, 0.1 mL lyophilized NCL-OCT2 F P (HIER)
7 mL Bond ready-to-use PA0532 P (HIER)
Oct-2 is a transcription factor belonging to the POU homeo-domain family
that binds to the Ig gene octamer sites regulating B cell specific genes. It is
dependent on the activity of B cell restricted coactivators such as BOB.1/
OBF.1. Oct-2 protein expression is not restricted to B cells, although
expression levels are much higher in these cells. Reports indicate that
germinal center B cells shows higher expression for Oct-2 and BOB.1/OBF.1.
In addition, Oct-2 expression is reported to be significantly greater in
germinal center derived lymphomas, although other B cell lymphomas also
display high levels of expression. Reed Sternberg (RS) cells represent the
malignant cells in classical Hodgkin's disease and are derived from germinal
center B cells. In a number of these cases, cells do not express immunoglobulin
due to the presence of crippling mutations within the Ig genes. As Ig
gene expression in B cells also requires an interaction between octamer
sites and the transactivating factors Oct-2 and BOB.1, the absence of both
Oct-2 and BOB.1 expression represents a novel mechanism for
immunoglobulin gene deregulation in RS cells.
Refer to page 38 for the Bond ready-to-use format.
Human tonsil: immunohistochemical staining for Oct-2 gene product using NCL-OCT2.
Note intense nuclear staining of mainly germinal center B cells. Paraffin section.
Oct-3/4
Clone N1NK
1 mL, 0.1 mL liquid NCL-L-Oct3/4 P (HIER) W
7 mL Bond ready-to-use PA0934 P (HIER)
New!
Reference Range
Oct3/4 is a member of the POU homeodomain family of transcription factors,
which is expressed by embryonic stem cells and germ cells. A critical
amount of Oct3/4 levels are associated with loss of pluripotency. Oct3/4 has
been proposed as a useful marker for germ cell tumors which exhibit
features of pluripotentiality, including seminoma/dysgerminoma and
embryonal carcinoma, and establishing a germ cell origin for some
metastatic tumors of uncertain primary tumor.
Refer to page 38 for the Bond ready-to-use format.
Human seminoma: immunohistochemical staining for Oct-3/4 transcription factor using NCL-L-
Oct-3/4. Note intense staining of pluripotent tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies