Labelling Review row-Online

Primary Antibodies
Involucrin
Clone SY5
1 mL, 0.1 mL lyophilized NCL-INV F P (Enzyme)
Involucrin is a precursor (120 kD) of the epidermal cornified envelope which
becomes cross-linked during envelope assembly. Involucrin is expressed in
a range of stratified squamous epithelia, including the cornea which lacks a
distinct cornified layer and is expressed when differentiation is terminated.
In normal dermis, involucrin is expressed in the upper cornified layer.
However, in pathological conditions, involucrin expression is altered eg in
psoriasis and other benign epidermal hyperplasias, where involucrin
expression is found closer to the basal layer. Expression of involucrin is
abnormal in squamous cell carcinomas and premalignant lesions.
Product Specific Information
When using NCL-INV, enzyme pretreatment may enhance staining in some
cases.
Kappa Light Chain
Clone CH15 New!
1 mL, 0.1 mL liquid NCL-L-KAP-581 P (HIER)
Clone kp-53
1 mL lyophilized NCL-KAP FPW
Clone L1C1
1 mL, 0.1 mL lyophilized NCL-KAP-L1C1 F P (Enzyme)
Clone L1C1 is the main choice of end users for use on paraffin sections.
Immunoglobulins are polypeptides and comprise five major classes;
immunoglobulin G (IgG), IgA, IgM, IgD and IgE. Each immunoglobulin
consists of two identical heavy (H) chains and two identical light (L) chains.
These are also subdivided into sub classes eg IgG1. There are two classes
of light chain; kappa and lambda. The ratio of kappa chains and light chains
varies between Ig classes and sub classes, but is also species specific. In
humans, approximately 60 percent of light chains are kappa. However, in
any particular immunoglobulin molecule the light chain will be either kappa
or lambda. B cells contain either kappa or lambda mRNA.
Tonsil: immunohistochemical staining for Kappa Light Chain using NCL-L-KAP-581. Note
cytoplasmic staining of plasma cells. Paraffin section.
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Reference Range
Ki67 Antigen
Clone MM1
1 mL, 0.1 mL lyophilized NCL-Ki67-MM1 F P (HIER)
1 mL liquid NCL-L-Ki67-MM1 F P (HIER)
7 mL ready-to-use RTU-Ki67-MM1 F P (HIER)
7 mL Bond ready-to-use PA0118 P (HIER)
Clone K2 New!
7 mL Bond ready-to-use PA0230 P (HIER)
Polyclonal
0.2 mL lyophilized NCL-Ki67p F P (HIER)
The Ki-67 antigen is a human nuclear protein, which is expressed in all
active parts of the cell cycle (G1, S, G2 and mitosis), but absent in resting
cells (G0). In contrast to many other cell cycle-associated proteins, the Ki67
antigen is consistently absent in quiescent cells and is not detectable during
DNA repair processes. Thus, the presence of Ki67 antigen is strictly
associated with the cell cycle and confined to the nucleus, suggesting an
important role of this structure in the maintenance and/or regulation of the
cell division cycle.
Refer to page 32 for the Bond ready-to-use format.
Kip2 (p57 Protein)
Clone 25B2
1 mL, 0.1 mL lyophilized NCL-p57 P (HIER)
Cyclin dependent kinases are positive regulators of cell proliferation. p57
protein acts as a tumor suppressor to counter this. It is closely-related to
other CDKIs such as p21 protein (CIP1) and p27 protein (Kip1) as they share a
common structural N-terminal domain for binding to CDK/cyclin complexes
and inhibiting their kinase activity. Human p57 protein is found on
chromosome 11p15.5, a region which is reported to be a common site for
loss of heterozygosity in certain sarcomas, Wilms’ tumors and tumors
associated with the Beckwith-Wiedemann syndrome. There is increasing
interest in p57 as a marker in Gestational disease. Gestational trophoblastic
disease refers to a spectrum of proliferative disorders of the placental
trophoblast, with a wide range of histologic appearances and clinical
behaviors. Recent developments in changes in the criteria for histologic
diagnosis of these lesions due to earlier clinical diagnosis have been
reviewed Hui P et al., Advantages in Anatomical Pathology. 12(3): 116-125
(2005) and the ability to make more accurate diagnoses due to the
introduction of newer antibodies such as p57 is discussed.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range