Labelling Review row-Online

Primary Antibodies
Myosin Heavy Chain Antibodies
Clone S131
1 mL, 0.1 mL lyophilized Myosin Heavy Chain
(smooth muscle) NCL-MHC-Sm F P (HIER)
7 mL Bond ready-to-use PA0493 P (HIER)
Clone RNMy2/9D2
1 mL, 0.1 mL lyophilized Myosin Heavy Chain (developmental)
NCL-MHCd F
Clone WB-MHCf
1 mL, 0.1 mL lyophilized Myosin Heavy Chain (fast)
NCL-MHCf F
Clone WB-MHCn
1 mL, 0.1 mL lyophilized Myosin Heavy Chain (neonatal)
NCL-MHCn F
Clone WB-MHCs
1 mL, 0.1 mL lyophilized Myosin Heavy Chain (slow)
NCL-MHCs F
Myosin is a contractile muscle specific protein composed of two heavy and
four light chains. The myosin heavy chain has many isoforms which are
specific for different muscles or fiber types, some of which are
developmentally regulated. Smooth muscle myosin heavy chain (SM-MHC)
is a cytoplasmic structural protein that is a major component of the
contractile apparatus in smooth muscle cells. It has been reported to be
specific for smooth muscle development.
Refer to page 36 for the Bond ready-to-use format.
Human prostate: immunohistochemical staining for myosin heavy chain using NCL-MHC-Sm.
Note staining of smooth muscle fibers. Paraffin section.
/ 140
For detailed information on all products please visit our website:
www.leica-microsystems.com
Myotilin
Clone RSO34
1 mL lyophilized NCL-MYOTILIN F P (HIER)
The myotilin gene on chromosome 5q31 encodes a 498 amino acid
polypeptide with a molecular weight of 57 kD. Myotilin is a structural protein
of sarcomeric Z discs and sarcolemma in human skeletal and cardiac
muscle. It is homologous to palladin and titin in the two C-terminal Igdomains
and also to palladin in its unique serine-rich N-terminal region.
Myotilin interacts with alpha-actinin, actin and gamma-filamin. A missense
mutation in the myotilin gene is associated with limb-girdle muscular
dystrophy 1A (LGMD1A), an autosomal dominant disease characterized by
proximal limb weakness. It is highly conserved between human and mouse
with its expression being more widespread in the embryo than in the adult.
Expression of myotilin has been reported in adult skeletal and cardiac
muscle with variable expression reported in the peripheral nervous system,
lung, liver and kidney.
Human skeletal muscle: immunohistochemical staining for myotilin using NCL-MYOTILIN. Note
intense staining of muscle fibers. Paraffin section.
Napsin A
Clone IP64
1 mL, 0.1 mL liquid NCL-L-NapsinA P (HIER)
Napsin A has a specific function in normal alveolar epithelium and is
proposed to play a role in the proteolytic processing of surfactant
precursors. Napsin A is reported to be predominantly expressed in lamellar
bodies of type II pneumocytes, secondary lysosomes of alveolar macrophages,
respiratory epithelium of terminal and respiratory bronchioles,
plasma cells, within a subset of lymphocytes in normal lung as well as in
epithelial cells of renal tubules in normal kidney and is weakly expressed in
normal spleen. Past studies have also reported that Napsin A is expressed in
90 percent of primary lung adenocarcinomas.
Human lung adenocarcinoma: immunohistochemical staining for napsin A using NCL-L-Napsin
A. Note punctate cytoplasmic staining of malignant cells and infiltrating macrophages.
Paraffin section.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.
Reference Range