Labelling Review row-Online

Breast carcinoma showing neuroendocrine differentiation: immunohistochemical staining for
synaptophysin using NCL-SYNAP-299. Note cytoplasmic staining of tumor cells. Paraffin
section.
Synuclein, Alpha
Clone KM51
1 mL liquid NCL-L-ASYN P (HIER)
Refer to page 57 for further information about Alpha-Synuclein.
Tartrate-Resistant Acid Phosphatase
(TRAP)
Clone 26E5
1 mL, 0.1 mL lyophilized NCL-TRAP P (HIER)
7 mL Bond ready-to-use PA0093 P (HIER)
New!
Tartrate-resistant acid phosphatase (TRAP) is a basic, iron-binding protein
with high activity towards phosphoproteins, ATP and 4-nitrophenyl
phosphate. This isoenzyme has been reported through different applications
to be expressed in human alveolar macrophages, osteoclasts, spleen and
liver. Expression of TRAP is reported to be increased in the spleen and
monocytes of individuals with Gaucher's disease, Hodgkin's disease and the
sera of individuals undergoing active bone turnover. Elevated levels are also
reported to be associated with various B cell and T cell leukemias and
lymphomas, decidual cells, syncytiotrophoblasts and some macrophages
distributed throughout maternal and embryonic tissues.
Refer to page 41 for the Bond ready-to-use format.
Human osteoclastoma: immunohistochemical staining for tartrate-resistant acid phosphatase
using NCL-TRAP. Note intense cytoplasmic staining of osteoclasts. Paraffin section.
Tau
Clone Tau-2
1 mL, 0.1 mL lyophilized NCL-Tau-2 P (HIER)
The brain tissues from individuals with Alzheimer's disease are
characterized by an abundance of neurofibrillary tangles, neuropil threads
and abnormal neurites in senile plaques. Tangles represent dense accumulations
of ultrastructurally distinct paired helical filaments whose major
component is a microtubule-associated tau protein.
Product Specific Information
NCL-Tau-2, raised against the bovine tau protein, cross-reacts with the
phosphorylated form of human tau protein.
Human brain, Alzheimer's disease: immunohistochemical staining for tau protein using
NCL-Tau-2. Note intense staining of senile plaques and the surrounding dystrophic
neurites. Paraffin section.
Tenascin C
Clone 49
1 mL lyophilized NCL-TENAS-C F P (HIER+Enzyme)
Tenascin is a high molecular weight glycoprotein which has a unique
molecular structure containing domains homologous to epidermal growth
factor, fibronectin and fibrinogen. There are at least five members of the
tenascin family, tenascin C (TN-C), TN-R, TN-X, TN-Y and TN-W4. Tenascin
C was originally called tenascin. Tenascin itself was previously known as
myotendinous antigen and is thought to play a role in organizing the growth
of the extracellular matrix eg in wound healing. In addition, the presence of
tenascin on type III fibers on the inner periosteum and outer cortex of bone
appears to be important for normal osteogenesis. The expression of
tenascin is reported to correlate with cell proliferation and migration. Like
fibronectin, tenascin is a cell-substrate adhesive molecule that shares the
‘arginine-glycine-aspartic acid' sequence necessary for ligand recognition
by most integrins.
Product Specific Information
The heat induced epitope retrieval (HIER) technique followed immediately by 30
seconds of enzyme digestion produces optimal staining with NCL-TENAS-C.
Human breast carcinoma: immunohistochemical staining for tenascin using NCL-TENAS-C.
Note intercellular matrix staining around malignant cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies