Labelling Review row-Online

Primary Antibodies
c-erbB-3 Oncoprotein
Clone RTJ1
1 mL lyophilized NCL-c-erbB-3 F P (HIER) O
The c-erbB-3 oncoprotein is a member of the type 1 growth factor receptor
family which also includes c-erbB-2 and epidermal growth factor receptor
(EGFR). These receptors share a common overall structure consisting of an
extracellular domain, a transmembrane region and a cytoplasmic domain.
The expression of c-erbB-3 oncoprotein has been reported in chronic
pancreatitis, exocrine pancreatic cancer and in tumors of the gastrointestinal
tract.
Product Specific Information
NCL-c-erbB-3 recognizes an epitope in the cytoplasmic domain of the human
c-erbB-3 oncoprotein and does not cross-react with c-erbB-2 or EGFR. NCLc-erbB-3
may also be used in immunoprecipitation techniques.
Human rectal adenocarcinoma: immunohistochemical staining for c-erbB-3 oncoprotein using
NCL-c-erbB-3. Note cytoplasmic staining characteristic in these tumors. In other tumor types,
membrane staining is observed. Paraffin section.
c-fos Oncoprotein
Clone CF2
1 mL lyophilized NCL-FOS F
The c-fos proto-oncogene encodes a nuclear phosphoprotein and is a
regulator of transcription, forming a complex with the c-jun proto-oncogene
product. Expression of the c-fos gene is reported to be low in most adult
tissues, however, high levels of expression have been detected in normal
skin. A negative correlation between p53 protein expression and c-fos
oncoprotein expression has been reported in human basal cell carcinomas
of skin.
Checkpoint Kinase 1
Clone DCS-310.1
1 mL lyophilized NCL-Chk1 P (HIER)
Checkpoint kinase 1 (Chk1) is an evolutionary conserved protein, which in its
phosphorylated form regulates cell cycle progression in response to agents
that block DNA replication. Eukaryotic cells which are exposed to ionizing
radiation (IR) or other genotoxic stresses activate checkpoints to delay the
progression of the cell cycle. Defects in the IR-induced S phase checkpoint
causes radioresistant DNA synthesis, a phenomenon reported in cancerprone
individuals suffering from ataxia-telangiectasia. Both of the cell cycle
kinases, Chk1 and Chk2, act upstream of p53 in DNA damage responses. It
has been reported that Chk1 mutations have been implicated in the cause of
some cancers of families with Li-Fraumeni syndrome.
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Human tonsil: immunohistochemical staining for checkpoint kinase 1 using NCL-Chk1.
Note nuclear staining of proliferating cells. Paraffin section.
Cholecystokinin (CCK-8)
Polyclonal
0.25 mL lyophilized NCL-CCK-8p P (Enzyme)
See also CCK-8 (Cholecystokinin) on page 69.
Choline Acetyltransferase
Clone 38B12
1 mL lyophilized NCL-ChAT P (HIER)
Choline acetyltransferase (ChAT) is a 68 kD enzyme which catalyzes the
synthesis of acetylcholine (ACh) from choline and acetyl coenzyme A. The
human ChAT gene encodes two proteins, the 68 kD ChAT enzyme and a
27 kD protein immunologically related and coexpressed with ChAT in
cholinergic neurons of the central nervous system. The smaller protein may
play a role in the regulation of ACh synthesis. ChAT is expressed in
cholinergic neurons, the majority of the neurons in the nucleus basalis of
Meynert, large neurons in the striatum (putamen and caudate nuclei), the
majority of neurons in the pedunculopontine, hypoglossal, dorsal nucleus of
vagus and subgroups of neurons in Roller's and the medial olivary
accessory nuclei. Prominent staining is observed in ribonucleoprotein,
distributed at the periphery of large neurons of the nucleus basalis of
Meynert, the motor neurons in the hypoglossal and ambiguus nuclei.
Product Specific Information
NCL-ChAT does not label axons in the insular cortex of the internal capsule
non-cholinergic structures, endothelial cells or microglia.
Human brain, basal ganglia: immunohistochemical staining for choline acetyltransferase using
NCL-ChAT. Note cytoplasmic staining of neurons of the nucleus basalis of Meynert. Paraffin
section.
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This catalog is not for use in the USA.