Labelling Review row-Online

Tubulin Beta II
Clone KNY-379
1 mL lyophilized NCL-TUB-B2 P (HIER) W
Tubulin beta II is one of four subunits of tubulin. Tubulin is one of the most
widespread classes of multiprotein families where alpha and beta subunits
are used in the construction of microtubules. Studies in rats report that
tubulin beta II represents a significant proportion of beta tubulin expressed
in the adrenal gland, brain and testis. In fetal tissues, this isoform has been
reported in skeletal muscle as well as in brain. Expression has also been
reported in migrating neuroblasts, peripheral nerves, ganglion cells and
sensory organs of the developing rat nervous system and in developing
skeletal and smooth muscle cells, chondrocytes and vascular endothelia.
Product Specific Information
Clone KNY-379 does not cross-react with vimentin or nestin.
Human neuroblastoma: immunohistochemical tubulin beta II lipoprotein receptor using
NCL-TUB-B2. Note intense cytoplasmic staining of malignant cells. Paraffin section.
Tumor Necrosis Factor Receptor-
Associated Factor 1
Clone 7C11
1 mL liquid NCL-L-TRAF-1 P (HIER)
TNF receptor-associated factors (Traf) are a family of proteins that bind to
surface receptors forming signalling complexes with additional proteins that
mediate some cellular responses. Traf-1 can homodimerize or heterodimerize
with other Traf proteins leading to the activation of some
transcription factors such as nuclear factor kappa B and Jun-N-kinase. The
activation of nuclear factor kappa B is known to act in concert with
additional proteins to suppress TNF-alpha mediated apoptosis. The
expression of this protein is reported to be induced by Epstein-Barr Virus
(EBV).
Product Specific Information
NCL-L-TRAF-1 stains optimally when used in TBS-based wash buffer and
diluent systems.
Hodgkin’s Lymphoma: immunohistochemical staining for Traf-1 protein using NCL-L-TRAF-1.
Note intense membrane staining of Reed Sternberg cells. Paraffin section.
Tyrosinase
Clone T311
1 mL lyophilized NCL-TYROS F P (HIER)
1 mL liquid NCL-L-TYROS F P (HIER)
7 mL ready-to-use RTU-TYROS F P (HIER)
7 mL Bond ready-to-use PA0322 P (HIER)
The biosynthesis of melanin within melanocytes involves a family of
enzymes, a key member of which is tyrosinase. Tyrosinase deficiency is
associated with various forms of albinism and in particular oculocutaneous
albinism. L-tyrosine is the initial substrate for melanin biosynthesis and its
conversion to dopaquinone is catalyzed by tyrosinase whose expression is
reported in melanocytes and melanomas.
Refer to page 43 for the Bond ready-to-use format.
Human malignant melanoma: immunohistochemical staining for tyrosinase using RTU-TYROS.
Note cytoplasmic staining of tumor cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies