Labelling Review row-Online

Primary Antibodies
Granulysin
Clone RJT48
1 mL liquid NCL-L-Granulysin P (HIER)
Granulysin is a member of the Saposin-like protein (SAPLIP) family. It is a
lytic protein made up of two fragments, 15 kD and 9 kD, reported to be
expressed selectively by NK cells and activated T cells. Granulysin protein is
highly homologous to a porcine antimicrobial and antitumor protein called
NK lysine and to amebapores, polypeptides used by amoebae to kill
bacterial prey. Granulysin disrupts artificial liposomes and cell membranes,
damages mitochondria and activates caspase 9 to induce apoptosis in
nucleated cells.
Human NK cell lymphoma: immunohistochemical staining for granulysin using NCL-L-
Granulysin. Note intense granular cytoplasmic staining on a proportion of NK cells. Paraffin
section.
Granzyme B
Clone 11F1
1 mL, 0.1 mL lyophilized NCL-GRAN-B P (HIER)
1 mL liquid NCL-L-GRAN-B P (HIER)
7 mL ready-to-use RTU-GRAN-B P (HIER)
7 mL Bond ready-to-use PA0291 P (HIER)
Granzymes are neutral serine proteases which are stored in specialized lytic
granules of cytotoxic T lymphocytes (CTL) and in natural killer (NK) cells.
These CTL and NK cells are heavily involved in the elimination of neoplastic
and virally infected cells. Secretory granules containing perforin and
granzymes are instrumental in undertaking cytolytic activity. Granzyme B is
understood to enter a target cell through a perforin pore-formed channel to
induce DNA fragmentation and apoptosis. Granzyme B has also been
described in neoplastic CTL and NK cells.
Refer to page 30 for the Bond ready-to-use format.
Hodgkin's disease: immunohistochemical staining for granzyme B using NCL-L-GRAN-B.
Note granular cytoplasmic staining in a number of Reed Sternberg cells. Paraffin section.
/ 116
For detailed information on all products please visit our website:
www.leica-microsystems.com
Gross Cystic Disease Fluid Protein-15
Clone 23A3
1 mL, 0.1 mL lyophilized NCL-GCDFP15 P (HIER)
1 mL liquid NCL-L-GCDFP15 P (HIER)
7 mL ready-to-use RTU-GCDFP15 P (HIER)
7 mL Bond ready-to-use PA0350 P (HIER)
Gross cystic disease of the breast is a benign premenopausal disorder in
which cysts are a predominant pathological lesion. These cysts appear to
be formed from excessive apocrine cystic secretions. This fluid is composed
of several glycoproteins including a unique 15 kD monomer protein,
GCDFP15. It has been reported that cytosolic analysis of normal tissue from
all major organs has demonstrated GCDFP15 in apocrine epithelia, lacrimal,
ceruminous and Moll's glands and in numerous serous cells of the
submandibular, tracheal, bronchial, sublingual and minor salivary glands.
Cytosol from breast carcinoma lesions are reported to contain GCDFP15 at a
wide range of concentrations. The concentration is reported to be highest in
more differentiated carcinomas and GCDFP15 shows only a few positive
individual epithelial cells within lobules and small ducts in normal breast.
Expression has also been reported in fibroadenomas within areas of
apocrine metaplasia. GCDFP15 and prostate specific antigen are reported to
be co-expressed in androgen receptor-positive breast tumors.
Refer to page 30 for the Bond ready-to-use format.
Human breast, Paget's disease: immunohistochemical staining for gross cystic disease fluid
protein (15 kD) using RTU-GCDFP15. Note variable cytoplasmic staining of tumor cells. Paraffin
section.
H-CAM (CD44)
Clone DF1485
1 mL, 0.1 mL lyophilized NCL-CD44-2 F P (HIER)
See also CD44 (H-CAM) on page 79.
Products in this catalog are subject to regulatory approval.
This catalog is not for use in the USA.