Labelling Review row-Online

Gamma-Catenin
Clone 11B6
1 mL lyophilized NCL-G-CAT F P (HIER) W
Cell to cell adhesion is mediated by cadherins which form a complex with
catenins. Gamma-catenin or plakoglobin, is a major cytoplasmic protein of
82 kD that occurs in soluble and membrane-associated forms. The downregulation
of cadherins and catenins has been detected in many types of
human carcinomas and has been reported to be associated with tumor
progression. E-cadherin and the catenins are reported to be expressed in
normal epithelium as well as urothelium. E-cadherin plays a primary role in
the maintenance of epithelial integrity where its decrease or loss of
expression is reported to be strictly associated with neoplastic progression
in a variety of human carcinomas, including bladder carcinoma. The
combined decrease in expression of gamma-catenin, beta-catenin and Ecadherin
is found at the invasive front of both grade 2 and some grade 1
carcinomas, further supporting a link with the aggressive behavior of those
cancer cells. In thyroid carcinomas, catenins are also reported to be
downregulated at cell to cell junctions. Gamma-catenin expression is
reported to be partially or totally lost in the majority of papillary, follicular
and anaplastic thyroid carcinomas.
Human squamous cell carcinoma: immunohistochemical staining for gamma-catenin using
NCL-G-CAT. Note intense membrane staining of malignant epidermal cells. Paraffin section.
GAP43
Clone 1G7
1 mL lyophilized NCL-GAP43 P
Growth associated phosphoprotein 43 (GAP43) is a major protein of neuronal
growth cones and certain presynaptic terminals. It is a candidate for
involvement in both axon growth and synaptic plasticity. It has been
reported that in several neuronal systems, GAP43 expression is higher in
neurons that are extending axons, either during development or
regeneration of injured axons found in intact adult neurons. GAP43 is the
best characterized of the growth associated proteins and although its
normal action in vivo is unclear, GAP43 can bind calmodulin, inhibit
phosphatidylinositol phosphate kinase, be phosphorylated by protein kinase
C, affect neurotransmitter release and enhance filopodia in non-neuronal
cells.
Human brain, normal adult cerebral cortex: immunohistochemical staining for growth
associated protein 43 using NCL-GAP43. Note intense staining of neurons and apical
dendrites. Paraffin section.
Gastrin
Polyclonal
0.5 mL lyophilized NCL-GASp FP
7 mL Bond ready-to-use PA0681 P
Gastrin, a polypeptide hormone, occurs naturally in three forms: gastrin-14,
gastrin-17 and gastrin-34. Both primary and secondary G cell hyperplasia
are reported to be characterized by clustering of the immunoreactive cells
which sometimes project buds from the mucous glands.
Product Specific Information
NCL-GASp reacts with non-sulfated (I) and sulfated (II) gastrin-17 as well as
gastrin-34. The antibody cross-reacts with cholecystokinin octapeptide.
NCL-GASp labels gastrin or gastrin-analogue producing cells.
Refer to page 22 for the Bond ready-to-use format.
Normal human stomach: immunohistochemical staining for gastrin using NCL-GASp.
Note intense cytoplasmic staining of the gastric mucosa. Paraffin section.
Geminin
Clone EM6
1 mL liquid NCL-L-Geminin P (HIER)
New!
Geminin is a protein of 209 amino acids thought to be involved in the control
of DNA replication via the interaction with Cdt1. Geminin is not found in the
G1 phase of the cell cycle, but is first expressed in the G1 to S transition
phase, with expression levels rising through the rest of the cell cycle and
levels reaching a maximum during mitosis. It has been proposed that
Geminin may be a tumor suppressor protein. Geminin is reported to be
expressed in proliferating lymphocytes and epithelial cells eg germinal
centers in tonsil as well as in colon, spermatocytes, seminiferous tubules of
the testes, within the basal layers of the squamous epithelium of the skin
and breast. Geminin is reported to be upregulated in cancers such as non-
Hodgkin’s lymphoma, B cell lymphoma, breast carcinoma and colon
carcinoma.
Human chronic lymphocytic leukemia: immunohistochemical staining for Geminin using
NCL-L-Geminin. Note intense nuclear staining of proliferating neoplastic cells. Paraffin section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies