Labelling Review row-Online

Human seminoma: immunohistochemical staining for proliferating cell nuclear antigen using
NCL-PCNA. Note nuclear staining of proliferating tumor cells. Paraffin section.
Prostate Specific Antigen
Clone 35H9
1 mL, 0.1 mL lyophilized NCL-PSA-431 P
7 mL Bond ready-to-use PA0431 P (HIER)
Reference Range
Clone PSA 28/A4
1 mL liquid NCL-L-PSA-28A4 FP
7 mL ready-to-use RTU-PSA-28A4 FP
Clone 35H9 was developed to produce superior staining on paraffin sections.
Prostate specific antigen (PSA) is a 34 kD protein belonging to the kallikrein
family of serine proteases and was originally isolated and purified from
human seminal plasma. It was found to be immunologically identical and
biologically similar to a protein isolated from the prostate gland. PSA is
distinct from prostatic acid phosphatase. Low levels of expression of PSA
have been reported in non-prostatic tissues and tumors such as breast
carcinomas.
Refer to page 39 for the Bond ready-to-use format.
Human prostate gland: immunohistochemical staining for prostate specific antigen using
NCL-PSA-431. Note intense cytoplasmic staining of glandular epithelial cells. Paraffin section.
Prostate Specific Membrane Antigen
Clone 1D6
1 mL liquid NCL-L-PSMA P (HIER)
The prostate specific membrane antigen (PSMA) is expressed as a 750
amino acid glycoprotein but may also be found as PSM, a form of the protein
missing the first 57 amino acids. PSMA has two enzymatic activities, one as
a prostate-specific integral membrane folate hydrolase and the other as a
carboxypeptidase. Reports suggest that PSMA expression may correlate
with tumor burden and serve as an indicator of metastatic involvement. The
cellular localization of PSMA contrasts with that of prostate specific antigen
(PSA) and prostatic acid phosphatase (PAP) that are secreted proteins.
Human metastatic prostate carcinoma in liver: immunohistochemical staining for PSMA using
NCL-L-PSMA. Note cytoplasmic staining of tumor cells. Paraffin section.
Prostate Tumor Overexpressed Protein 1
Clone 17C6
1 mL lyophilized NCL-PTOV1 P (HIER)
The gene prostate tumor overexpressed protein 1, or PTOV1, was
discovered following the search for molecular markers of progression in
prostate cancer. The prostate in ageing males is highly susceptible to
benign and malignant proliferative changes. A number of genetic and
molecular alterations are responsible for the phenotypic conversion of
normal or hyperplastic prostate cells into malignant, invasive cells. In the
most common form of prostate cancer, carcinomatous cells arise as
multifocal lesions against a background of hyperplastic tissue, called the
zone of benign peripheral hyperplasia. PTOV1 is reported to be expressed at
significantly higher levels in prostate cancers than in benign prostatic
hyperplasia or in normal prostate tissue. In addition, PTOV1 protein is
overexpressed in premalignant cells from prostatic biopsies such as those
with prostate intra-epithelial neoplasia and in advanced-stage prostatic
cancer cells. PTOV1 protein is located mainly in the cytoplasm with
juxtanuclear positivity in tumor cells. The detection of a 1.8kb PTOV1
transcript has been reported in normal human brain, heart, skeletal muscle,
kidney and liver and at lower levels in normal prostate.
Human prostatic carcinoma: immunohistochemical staining for prostate tumor overexpressed
protein 1 using NCL-PTOV1. Note membrane staining of prostatic neoplastic cells. Paraffin
section.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies