Labelling Review row-Online

CD10
Clone 56C6
1 mL, 0.1 mL lyophilized NCL-CD10-270 F P (HIER) W
1 mL liquid NCL-L-CD10-270 F P (HIER) W
7 mL ready-to-use RTU-CD10-270 F P (HIER)
7 mL Bond ready-to-use PA0270 P (HIER)
CD10 antigen, also called neprilysin, is a 100 kD cell surface
metalloendopeptidase which inactivates a variety of biologically active
peptides. It was initially identified as the common acute lymphoblastic
leukemia antigen (CALLA) and was thought to be tumor-specific.
Subsequent studies, however, have shown that CD10 antigen is expressed
on the surface of a wide variety of normal and neoplastic cells. In other
lymphoid malignancies, CD10 antigen is reported to be expressed on cells of
lymphoblastic, Burkitt's and follicular lymphomas. CD10 antigen has been
identified on the surface of normal early lymphoid progenitor cells, immature
B cells within adult bone marrow and germinal center B cells within
lymphoid tissue. It is also expressed in various non-lymphoid cells and
tissues, such as breast myoepithelial cells, bile canaliculi, fibroblasts, with
especially high expression on the brush border of kidney and gut epithelial
cells. (G. McIntosh et al. American Journal of Pathology. 154(1): 77-82
(1999)).
Refer to page 19 for the Bond ready-to-use format.
Human lymphoblastic lymphoma: immunohistochemical staining for CD10 antigen using
NCL-CD10-270. Note intense membrane staining of neoplastic lymphoid cells. Paraffin section.
CD11b (Mac-1)
Clone 44
1 mL lyophilized NCL-CD11b F
Reference Range
The CD11b (Mac-1) leukocyte associated protein consists of two polypeptide
chains with molecular weights 95 kD and 170 kD. Mac-1 functions as
a cell surface receptor for the C3bi complement fragment. It belongs to a
family of heterodimeric proteins (designated CD11) which share a common
beta chain CD18 (95 kD). The alpha chain component (CD11b) of the
leukocyte associated protein is expressed on granulocytes, monocytes,
macrophages and NK cells.
CD11c
Clone 5D11
1 mL, 0.1 mL liquid NCL-L-CD11c-563 P (HIER)
7 mL Bond ready-to-use PA0554 P (HIER)
CD11c is a member of the leukocyte integrin family of adhesion proteins. It is
reported to be expressed in normal tissues, mainly on myeloid cells eg in
bone marrow myelocytes, premyelocytes, metamyelocytes, non-segmented
and segmented neutrophils with high levels reported on tissue macrophages
and monocytes and with lowest levels in granulocytes. It is also reported to
be expressed on NK cells, activated T cells, lymphoid cell lines, including
hairy cell leukemias and a proportion of interdigitating dendritic cells. CD11c
antigen expression is reported to be a useful indicator of monocyte
differentiation in the classification of acute myeloid leukemias and for the
identification of hairy cell leukemias.
Refer to page 19 for the Bond ready-to-use format.
Human hairy cell leukemia: immunohistochemical staining for CD11c antigen using
NCL-L-CD11c-563. Note membrane staining of malignant cells. Paraffin section.
CD13
Clone 38C12
1 mL, 0.1 mL lyophilized NCL-CD13-304 P (HIER)
Reference Range
CD13 antigen, also known as aminopeptidase N, is a member of the type II
integral membrane metalloproteases which also includes the leukocyte
antigens CD10, CD26, CD73 and BP-1. CD13 antigen is a receptor for the
coronaviruses which cause respiratory disease in humans and several
animal species. The antigen functions as a zinc-binding metalloprotease
which plays a role in cell surface antigen presentation by trimming the Nterminal
amino acids from MHC class II-bound peptides. CD13 antigen is
reported to be expressed on granulocytes, monocytes and their precursors,
most acute myeloid leukemias and a smaller proportion of acute lymphoid
leukemias. Non-hematopoietic cells which express CD13 antigen include
epithelial cells, renal proximal tubules, intestinal brush border, endothelial
cells, fibroblasts, brain cells, bone marrow, osteoclasts and cells lining the
bile canaliculi.
F Frozen I Immunofluorescence E Electron microscopy
P Paraffin C Flow cytometry O Other applications
W Western blotting
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Primary Antibodies