Angelika Semmler - KOPS - Universität Konstanz

10 Anhang
10.1.2 Peptid 63 (Trp-Xaa Sollbruchstelle)
Cyclo[Trp-Gly-His-Pro-Gln-Glu(bAla-bAla-Phe(4Br)-Arg-Hsl)] (63→ 64)
Analytische RP-HPLC (Nucleosil Säule): (45-100% B in 20 min, 1 ml/min) tR = 11,1 min
ESI-MS: 1397,9 m/z [M+H] +
Berechnet: C61H83BrN19O16 + (1398,53 m/z)
250
200
150
100
50
0
-50
-100
HPLC-Chromatogramm
mAU
300
214nm,4nm (1.00)
172
0
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 min
ESI-IT-Massenspektrum
10.965 11.133
H 2N
13.672
O
HN
O
O
N
H
N
N
O
H
N
O
N
H
NH O
O
NH
18.054
HN
NH
O
O
H
N
O
H
N
O
H
N
+
Chemical Formula: C61H81BrN19O15 Exact Mass: 1398,5337
Molecular Weight: 1400,3176
O
N
H
H 2N NH 2
O
Br
NH
H
N
%
B.Conc.(Method)
O
O
90
80
70
60
50
40
30
20
10