Abstract 230 - IMPIEGO DEI GLICOPEPTIDI NELLA ... - SIMIT

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10° CONGRESSO NAZIONALE SIMIT
Abstract 206
- MODERATE CONCORDANCE BETWEEN ABBOTT REAL-TIME HIV-1 ASSAY AND
ROCHE COBAS/TAQMAN HIV-1 V2.0 PROCEDURE FOR THE QUANTIFICATION OF
HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1) RNA AT LOW LEVEL -
Amendola A.* [1] , Marsella P. [1] , Bloisi M. [1] , Capobianchi M. R. [1]
- [1] INMI "Lazzaro Spallanzani" ~ Roma
INFEZIONI DA HIV
Premessa: There is increasing interest about HIV-1 RNA quantification in samples from
virologically suppressed patients with HIV-1 RNA below 40cp/ml. It has been described
that, at the lower limit of quantification, concordance between commercial assays is weak.
Obiettivo: We compared the performance of Abbott Real-time HIV-1 assay and Roche
COBAS/TaqMan HIV-1 v2.0 for quantification of HIV-1 RNA in clinical samples with viral
load values ranging from 40 to 500cp/ml. Moreover, the concordance on the ability to
detect the presence of HIV-1 RNA in samples resulted below 40cp/ml with the Abbott
assay was also analysed.Quantitative results were compared using correlation, linear
regression and Bland&Altman analyses of log10-transformed HIV-1 RNA copy numbers.
Concordance on the ability to detect HIV RNA was measured by Cohen‟s kappa statistics.
Risultati: A good correlation between assays was observed in samples with viral load
values ranging from 40 to 500 cp/ml (Pearson r=0.8540; p=0.0001): Abbott and Roche
assays measured a mean value of viremia of 2.003 + 0.376 log10cp/ml and 2.038 + 0.240
log10cp/ml, respectively, and quantitative differences did not exceed 0.3 log10cp/ml.
Below 40cp/ml, the degree of concordance between the two assays for samples given as
“detected