ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

Creating an Instrument Control Method 101
Lamp OFF at the end of run
The option is disabled for the K-2700 detector. Check the Lamp OFF box
to switch the detector lamp off after a measurement is finished. This
option may be used for the shutdown methods.
You should switch the lamp on from the direct control window
before you start the next working session. After power up, allow 30
minutes for the lamp to stabilize before running samples that may
require sensitive measurements.
Advanced
Pressing the Advanced button opens the window for reference
correction:
Fig. 149 reference correction window
Referring to a wavelength with practically zero absorption the drift due for
instance to a too short heating time of the lamp can be eliminated.
Reference Correction
According to the configuration up to 4 wavelengths can be monitored
from the 3D data stream. Select the checkbox to activate the associated
channel.
Sample
The sample wavelength is defined in the Acquisition tab.
Bw
The bandwidth for the sample channels are also defined in the
Acquisition tab.
Ref
Reference is the wavelength at which a reference absorbance is
measured as a function of time. The reference wavelength
compensates for fluctuations caused by changes in baseline
absorbance, for example due to changes in solvent composition
during gradient elution. The selected wavelength must be higher than
the sample wavelength and be inside of the scan range for this
detector.
Bw
Enter the bandwidth of the reference wavelength. Limits are 1 to 32
nm in 1 nm steps.
Scan, nm [Range xxx – yyy nm]
The displayed range depending on the detector type and the installed
lamps will only be updated, if you open that window online.