ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 267
Fig. 378
SEC Setup
Dist. Trace will appear in a separate window. Notice the axes are
Molecular Wt. Vs Slice Area for this trace.
SEC Setup involves entering Molecular Mass information about your
standard, which will subsequently be used to calculate Molecular Mass
numbers for your unknown samples. SEC baselines, result ranges, and
peaks can be entered automatically with graphic programming using a
stored calibration standard. These values can also be entered manually.
You must then complete the SEC Setup by entering the information
required for the selected SEC calibration method and calculations.
The information displayed in the SEC Setup screen will vary depending
on the Calibration Type selected (Narrow, Broad1, Broad2, or Universal).
For details, see the section describing the calibration method of choice.
When you have completed the SEC Setup, you are ready to acquire and
process your SEC runs. You can acquire data either manually (single
injection), or by batch acquisition (auto injector). See the ChromGate ®
User’s Guide for information on creating a batch sequence.
SEC Setup Narrow Standard Method
When you select the Narrow Standard method for Calibration Type, the
SEC Setup screen will look similar to the one displayed below.