ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

Short guide ChromGate®, KNAUER Instrument Control 19
To use this feature, select the Baseline Check option in the Single
Run dialog box or include Baseline Check in the current Sequence
line.
When the baseline feature is selected, the initial conditions for the run will
be used to acquire baseline data. If the data is satisfactory for all
channels, the run will be performed; if the baseline data is not
satisfactory, the run will be aborted and a message to that effect will be
presented.
Trigger tab
The Trigger tab is used to indicate the action to start a chromatogram.
The definition of the various options is presented on the tab. For details
see page 135.
Quantification of the Compound(s) of Interest
Overview
Integration
The change of the signal from a detector is related to the concentration of
the eluting compound. Under ideal circumstances, the peak for each
compound of interest will be well-resolved from other peaks in the sample
and will be a Gaussian peak. In this case, the area of the peak is directly
related to the concentration of the compound of interest. The analyst
could generate a calibration plot using standards of known concentration
to determine the concentration/area relationship, and then use the
calibration plot to determine the concentration of unknowns.
The calibration data is incorporated into the method, so that when you
select a method to use, all of the information that is required for
quantization is loaded.
Integration of the chromatogram requires two basic parameters, the
width and the threshold, which define the starting point and ending point
for a peak and to distinguish a peak from noise. When a method is
established, the default values are entered, and these can be edited via
the Integration Events window which is accessed by selecting
Method/Integration Events.
Fig. 13 Integration Events window
The Width value is used to calculate a value for bunching or smoothing
the data points before the integration algorithm is applied integration
works best when 20 points are sampled across a peak. The width should
be selected for the narrowest peak in the chromatogram.
The Threshold value is the first derivative of the chromatogram, and is
used to distinguish the peak from noise and/or drift.
To set the noise and drift, select the desired event field, then select the
start and stop time and value.
ChromGate ® includes a large number of additional parameters that can
be used to refine the chromatogram. These can be applied on a
programmed basis or on a manual basis for data that is already collected.
A detailed discussion on integration is presented in the ChromGate ®
Chromatography Data System, Reference Manual.