ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

SEC Option 277
Fit Type
Select a calibration curve fit type from the drop-down list. The choices
include: Point-to-point, Linear, Quadratic, Cubic, 4th Degree Polynomial,
and Spline.
Goodness of Fit
The calibration curve for the data in the SEC Setup will be displayed in
the calibration curve window. The r 2 value, which represents goodness
of fit of the calibration points to the curve selected, will be displayed
above the curve. A value of 1.00 indicates perfect fit. Select the Time or
Volume button at the bottom of the calibration curve to display the x-axis
of the curve as either retention time in minutes or retention volume. If
you select volume, enter the flow rate in ml/min.
Remove calibration curve outliers
To temporarily remove an outlier from the calibration curve, click on the
point with your mouse. It will change color and the curve will be recalculated
with the point removed.
Time/Volume
Select the mode of viewing for your calibration curve: Time or Volume. If
you select Volume, you must enter the flow rate so that the curve is
displayed correctly.
Print
Click this button to print your current SEC method information.
Export settings
Click this button to select export options and path.
When you have completed the SEC Setup, you are ready to acquire and
process your SEC runs. You can acquire data either manually (single
injection), or by batch acquisition (auto injector). See the ChromGate ®
User’s Guide for information on creating a batch sequence.
Zooming on the Calibration curve
You can zoom on the SEC Calibration curve by clicking and dragging
with the mouse. Double-click the mouse in the graph area to unzoom the
curve view.
Once you have zoomed in on a chromatogram, you can scroll the
chromatogram to the right or left without losing the zoom. This is done by
pressing the keys down and moving the mouse until
the cursor changes to a "hand" and dragging it to the left or right.
You can also scroll the X- or Y- axis to view features which may be out of
the range. To do this, press the keys down while the
mouse cursor is outside the graph area, yet near the axis of interest. The
cursor will change to an up/down arrow near the Y-axis, or a left/right
arrow near the X-axis. Moving the mouse in this mode will scroll the
graph up/down or left/right on the axis.
To restore the original view, do a right-hand mouse click in the
chromatogram window, followed by the Full Unzoom command.
Removing Calibration Outliers
You can temporarily remove an outlier from your calibration curve by
clicking on the outlier point on the calibration curve. When you select an
outlier point this way, the point will turn red, as will its Molecular Mass