ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

28 Short guide ChromGate®, KNAUER Instrument Control
Collecting Data
General Data Collection Instructions with ChromGate
The user should review the operating manual for each component in
the system for warm up requirements, preparing the component for
operation, etc.
This section includes requirements and suggestions relative to the
computer and e-line network when ChromGate ® is used to operate the
HPLC system.
Before starting the chromatographic runs:
� Make sure that the energy saving feature and the screen saver
on the computer are off.
� Check that all modules are connected to the e-line cable
� To power up the system, first turn on the PC and finally power up
the various modules.
If a unit loses power or if the communication is broken, turn off all
components and start over.
Collecting Data
� If a module has been operating via the local mode (UI interface),
make sure that it is not in use when connecting the cable.
� If you are using a manual injector, make sure that the system is
in the waiting for injection mode waiting for trigger before
injecting.
� If you are using an autosampler with a single rack, the input
numbers should be integers. If you are using multiple racks, input
vial numbers, using a letter and number (e.g. A1, B3, etc.) should
be used. If an incorrect vial number or invalid volume is noted,
the sequence will stop.
When you want to collect data, click on the desired instrument to present
the Instrument Wizard dialog box (Fig. 9 on page 16) and select the
desired operation.
If you select Run one sample, the Single Run Acquisition dialog box will
be presented. Enter the desired Sample ID, method and name (e.g.
sample.dat) and then press Start.
The chromatogram will be collected as described in the method. During
the data acquisition, the chromatogram will appear in the window as
shown below.