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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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50 Setup and Control of Knauer HPLC Systems<br />

All LAN-controlled devices can be added and configured<br />

automatically using the Auto Configuration button on the<br />

configuration main window. Beside this, you can let the software<br />

search for your already connected and switched-on device from the<br />

devices configuration window. The option “Use S/N to identify the<br />

instrument” must be enabled. Click on the button to start the<br />

search for all connected LAN devices of the currently configured<br />

type. If more than one will be found, all hits will be shown in the<br />

window. Select the desired device from the shown list. The software<br />

will read-out the information as serial number and will fill it<br />

automatically into the configuration screen. For all Knauer LAN<br />

devices port 10001 is selected by default. Don’t change this setting,<br />

otherwise no communication can be established. To search for an<br />

instrument, the device must be connected, switched on and must<br />

have a valid IP address.<br />

As an option, you can enter the IP address of your device, if the<br />

option “Use S/N to identify the instrument” is disabled. Please note,<br />

that in case the device receives the IP address from a DHCP server,<br />

e.g. a router, the IP address may change if the device is switched off<br />

and switched on.<br />

Channels (available not for all detectors)<br />

For multi-wavelength detectors more than one channel can be activated.<br />

According to the selected number, the channel fields become accessible<br />

for editing. The channel name will be shown later in the method window<br />

and in the result reports. Only channels, which have been activated in the<br />

detector configuration, will be available in the method setup later for data<br />

acquisition.<br />

Serial Number<br />

If you have read-out the device via LAN, the serial number has been<br />

added automatically. Otherwise type in the serial number of the detector<br />

you are using. The serial number of network controlled detectors is used<br />

for the device identification and addressing during communication. This<br />

serial number must match the serial number of the defined pump. If not,<br />

you will get an appropriate error message if you later try to start in run.<br />

Y-Axis Units<br />

Select the type of units to be displayed on the y-axis of your<br />

chromatogram from the drop-down list. For the UV and diode array<br />

detectors the choices are AU (Absorption Units), mAU (0.001 AU) or<br />

µAU (0.000001 AU). For the RI detectors, the only choice is µRIU and for<br />

the spectrofluorometric detector the units are given as a.u. (arbitrary<br />

units).<br />

For DAD’s you have additionally also the choice ADC counts.<br />

Selecting this, the signal mode in the Instrument setup (page 102)<br />

will be indicated as ‘Intensity’ without access for changing it. This<br />

change you can perform in the configuration window only.<br />

Flow Cell<br />

Enter a description for the flow cell you are using. The description will be<br />

printed out when you print the instrument configuration.<br />

Add. Info<br />

Click the button Add. Info... to enter more detailed description of the<br />

detector.

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