ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

ChromGate® System Suitability Setup 185
ChromGate ® System Suitability Setup
Fig. 278
Before ChromGate ® can make System Suitability calculations, you must
enter the required acceptance ranges for the peaks of interest. This is
done in using the Method – System Suitability command.
1. Click on the Method – System Suitability command to view the
System Suitability Setup dialog.
2. Select the first peak to be used for calculations by highlighting it with
the mouse in the Compounds list.
3. In the adjacent spreadsheet, click on the first field in the Parameter
column. A drop-down list of available parameters is displayed. Select
a parameter from the list. If you do not wish to perform a suitability
test on any given peak, simply leave the Parameter fields blank.
Similarly, if you do not wish to have one of the test criteria used (for
example, %RSD), leave it blank. Some parameters have a choice of
calculation methods. These parameters have the calculation method
displayed after the parameters in parenthesis (e.g. Plates/Meter (JP)
indicates Plates/Meter calculated using the Japanese Pharmacopeia
calculation). For details on these calculations, see the equations
section.
4. For each parameter selected, enter a minimum value (Min),
maximum value (Max), and maximum allowed percent relative
standard deviation (%RSD).
5. If you want a noise test to be performed, click on the lower
spreadsheet in the column labeled Test. Select one of the noise
calculations Noise (rms)/ASTM Noise (rms), or 6-sigma Noise
(rms) from the drop-down list. If you select this box, you must enter a
Start and Stop time for the test, and the Threshold value to
determine acceptable limit. The RMS noise value for the portion of
chromatogram between the Start and Stop times will be calculated
and compared to the Threshold value to determine whether the test
Passed or Failed. Note that the times you enter for the noise test
should be representative of a baseline area of your chromatogram
where not peaks elute. Keep in mind, that the noise may depend on
the detector type, wave length, flow rate, solvent and other
parameters used in the test.