ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

ChromGate® PDA Option 221
Peak Table
3 Point Purity
Checking this indicates that 3 point peak purity should be printed to the
right of the spectrum graph.
Similarity
Checking this indicates that peak apex similarity to the reference
spectrum should be printed to the right of the spectrum graph. The use of
this value requires that a reference spectrum be included in the peak
table. See the section on Analysis and Calculations for details.
Spectral Display
The controls in this group specify the content and labeling of the
spectrum graphs.
Show Lambda Maxima
Checking this box will annotate each spectrum graph with the 3 largest
lambda max absorbance.
Show Lambda Minima
Checking this box will annotate each spectrum graph with the 3 largest
lambda minima absorbance.
Background Correction
Checking this will cause each apex spectrum to be corrected for
background using the chromatographic baseline prior to being used
elsewhere in analysis. Refer to the PDA Analysis and Calculations
section for details on the formula used.
Filtering Type
This allows the user to specify a mathematical filtering function to be
performed on all spectra extracted from the 3D data during analysis.
Refer to the PDA Analysis and Calculations section for details on the
formula used for each filter.
Graph Height
This specifies the relative height of the spectrum graph. A value of 100%
corresponds to a standard sized graph. A larger value may be selected to
provide more a detailed plot.
Graph Properties
Pressing this button displays the graph properties dialog as found on the
standard Spectrum graph.
When using the PDA option, certain columns will be added to the peak
table that enable you to analyze peaks from the PDA detector.
Detection
Select the basis for the identification of the peak. If you choose Ret Time,
only the retention time will be used for identification of the peak. If you
choose Ret Time with Spectral Confirm, the Similarity of the peak’s
spectrum to that of a designated reference spectrum will be used in
addition to the retention time as the basis of peak identification.
Spectrum
If you want Similarity to be used as a basis for peak identification, then
click on the arrow to the right of this field to specify the stored reference
spectrum to be used for comparison. During identification, this reference
spectrum is compared to the peak apex spectrum and a similarity index is