ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

272 SEC Option
All samples analyzed with a Universal Calibration must have the
Mark-Houwink constants or intrinsic viscosity specified here. If you
analyze samples with different values, you must create a new
method with the values for those samples.
Reference Peak Time (min)
Select this box if you are using a retention time reference peak to adjust
for changes in flow rate. All elution times/volumes used in calculations
will be offset by the time difference between the value entered here and
the time of the reference peak in the sample. Enter the expected
retention time for the reference peak, in minutes.
Reference Window (%)
If you are using a reference peak, enter a value for the % window for the
reference peak. A peak falling within +/- this % of the expected retention
time for the reference peak will be considered the reference peak. (If two
peaks fall within the window, the peak closest to the center will be chosen
as the retention time reference.)
Fit Type
Select a calibration curve fit type from the drop-down list. The choices
include: Point-to-point, Linear, Quadratic, Cubic, 4th Degree Polynomial,
and Spline.
Goodness of Fit
The calibration curve for the data in the SEC Setup will be displayed in
the calibration curve window. The r 2 value, which represents goodness
of fit of the calibration points to the curve selected, will be displayed
above the curve. A value of 1.00 indicates perfect fit. Select the Time or
Volume button at the bottom of the calibration curve to display the x-axis
of the curve as either retention time in minutes or retention volume. If
you select volume, enter the flow rate in ml/min.
Remove calibration curve outliers
To temporarily remove an outlier from the calibration curve, click on the
point with your mouse. It will change color and the curve will be recalculated
with the point removed.
Time/Volume
Select the mode of viewing for your calibration curve: Time or Volume. If
you select Volume, you must enter the flow rate so that the curve is
displayed correctly.
Print
Click this button to print your current SEC method information.
Export settings
Click this button to select export options and path.
When you have completed the SEC Setup, you are ready to acquire and
process your SEC runs. You can acquire data either manually (single
injection), or by batch acquisition (auto injector). See the ChromGate ®
User’s Guide for information on creating a batch sequence.