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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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Creating an Instrument Control Method 125<br />

Fig. 178 Autosampler Triathlon editing user defined programs, new step<br />

Select the Action from the drop-down list. The appearance of the dialog<br />

will be changed, depending on the selected action.<br />

Fig. 179 Autosampler Triathlon UDP action choices<br />

Aspirate<br />

Aspirates a programmed volume from a sample vial, a destination<br />

vial, a wash vial, or one of the reagent vials into the buffer tubing.<br />

Speed and height of the syringe can be entered. The maximum<br />

volume that can be aspirated is the total volume of the syringe. If more<br />

than one consecutive aspirating action (from different sources) is<br />

programmed, the total volume of the consecutive aspirating actions<br />

cannot exceed the volume of the syringe.<br />

Dispense<br />

Dispenses a programmed volume from the buffer tubing into the<br />

sample vial, a destination vial, the waste, a wash vial, or one of the<br />

reagent vials. Speed and height of the syringe can be entered.<br />

Syringe Valve<br />

This action controls the inter-connections of the syringe to one of its<br />

three ports:<br />

NEEDLE: connection to the sample needle.<br />

WASH: connection to the wash solvent bottle.<br />

WASTE: connection to the syringe waste tubing.<br />

Syringe<br />

This action controls the movement of the syringe<br />

LOAD: loads the syringe with the programmed volume.<br />

UNLOAD: unloads the syringe with the programmed volume.<br />

HOME: initializes the syringe to its HOME position. The volume<br />

previously aspirated will be dispensed to the last<br />

programmed position.<br />

Needle Wash<br />

With this action a needle wash can be performed. The content of the<br />

buffer tubing will not be rinsed to waste before the washing procedure

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