ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

Creating an Instrument Control Method 103
the noise because for each positive noise excursion there is a good
likelihood that it will be cancelled by a negative excursion. But since the
signal is not random, it will remain the same over multiple measurements.
A high bandwidth value will decrease the noise level but also decrease
the possible resolution which can be measured. The default value is 8 nm
but if your peaks have substantially wider UV spectra you can increase
the bandwidth value in ChromGate to obtain a lower noise level.
Each discrete channel can use its own wavelength program and can be
measured with its own bandwidth, so you can always obtain the lowest
noise conditions while still having a low enough bandwidth to not degrade
the resolution measured.
This means that the diode array detectors can always be optimized for
maximum performance. The scan parameters can be set to the values
which are optimal for doing the scans, and yet the discrete channels can
be measured independently at their optimal values.
The wavelength table is a spreadsheet to enter your time (detector)
program. In the first row (at time 0.00 min), enter the initial wavelength(s)
in nm. The editing principle including programming the digital outs
(S 2600 only) is the same as for the time (gradient) program, described in
the chapter Instrument Setup – Pumps, Pump Program.
Instrument Setup – Fluorescence Detector RF-10Axl / RF-20A
The Fluorescence Detectors RF-10Axl and RF-20A/Axs support the
general detector settings described on page 91.
The detector RF-10Axl only supports a (fixed) sampling rate of 0.5
Hz. For higher sampling rates the data acquisition via the 1V
integrator output and an A/D converter (as Knauer interface box IF2)
must be used.
The detector RF-20A/Axs supports sampling rates up to 0.5 Hz. For
higher sampling rates the data acquisition via the 1V integrator
output and an A/D converter (as Knauer interface box IF2) must be
used. The serial control only allows the same features as for the
detector RF-10Axl. All advanced features cannot be supported.
Please be sure that the detector has a firmware version that allows
for serial control.
Fig. 152 Fluorescence Detector RF-10Axl setup window
Lamp OFF at the end of run
Check the lamp OFF box to switch off the detector lamp after a
measurement is finished. This option may be used for the shutdown
methods. This option is supported only for detector firmware versions
3.32 and higher.
All additional settings are integrated as additional columns into the
spreadsheet for the detector time program. The creation of the detector