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ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

ChromeGate 3.3.2 Software Manual - KNAUER Advanced Scientific ...

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Creating an Instrument Control Method 99<br />

Fig. 144 Detector S 2550 Scan setup window with PDA option<br />

A click on the <strong>Advanced</strong>… button will open the following dialog box.<br />

Fig. 145 Detector S 2550 <strong>Advanced</strong> setup window<br />

Scan if a peak is detected<br />

At any detected peak the software will perform a scan automatically if this<br />

option is activated. All further settings in this area are valid for the whole<br />

method. You may decide whether the scan shall be performed At the<br />

peak apex or At the beginning of the peak with the given Delay [min].<br />

With the Slope [µAU/sec]: and at Height [µAU]: settings you can define<br />

the thresholds, necessary for evaluating the signal as a peak. The unit<br />

[µAU] depends on the configuration setting.<br />

Use channel defines the data channel where the analysis is performed.<br />

You cannot change this selection during a run. However you can change<br />

the measuring wavelength of this channel as often as necessary.<br />

The option Continuous quick scan cannot be enabled. This option is<br />

not available at this time.<br />

Warning! While scanning, the data acquisition of the detector is<br />

interrupted. This leads to a plateau shape for that time and thus to a<br />

false quantitative result. For accurate and reliable qualitative and<br />

quantitative results the run should be performed twice, with and<br />

without scanning.<br />

“Scan” column in time table<br />

If the option “Acquire 3D data” is enabled, a new column “Scan” will be<br />

available in the time table for the detector S 2550. If you want to perform<br />

a time-defined scan, enter a new line into the table with the desired time<br />

and enable the scan by clicking the check box. Please note, that during a<br />

scan the measurement signal(s) cannot be acquired. In the<br />

chromatogram you will find a plateau for the scanning time. It is not<br />

allowed to enable a scan in the first and last line of the time table. The<br />

time between two scan must be 0.1 min in minimum.

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